The development of energy-efficient solutions for large-scale fermenters demands a deep and comprehensive understanding hydrodynamic heat mass transfer processes. Despite wide variety research dedicated to measurements intensity in bubble flows, this subject faces new challenges due the topical innovative bioreactor designs. In order understand fluid dynamics gas–liquid medium, researchers need...

Talaromyces albobiverticillius 30548, a marine-derived fungus, produces Monascus-like azaphilone red/orange pigments which have the potential for various industrial applications. The objective of this study was to scale up pigment production in 2 L bioreactor with working volume 1.3 media and compare its biomass growth against small (500 mL) shake flasks 200 mL volume. Additionally, fungal morp...

We report the key findings from numerical solutions of a model of transport within an established perfusion bioreactor design. The model includes a complete formulation of transport with fully coupled convection-diffusion and scaffold cell attachment. It also includes the experimentally determined internal (Poly-L-Lactic Acid (PLLA)) scaffold boundary, together with the external vessel and flow...

Conventionally, some bioartificial liver devices are used with separate plasmapheresis unit to separate out plasma from whole blood and adsorbent column to detoxify plasma before it passes through a hepatocytes-laden bioreactor. We aim to develop a hybrid bioreactor that integrates the separate modules in one compact design improving the efficacy of the cryogel based bioreactor as a bioartifici...

BACKGROUND Heterologous production of natural products in Escherichia coli has emerged as an attractive strategy to obtain molecules of interest. Although technically feasible most of them are still constrained to laboratory scale production. Therefore, it is necessary to develop reasonable scale-up strategies for bioprocesses aiming at the overproduction of targeted natural products under indu...

BACKGROUND Pichia pastoris is a widely-used host for recombinant protein production; expression is typically driven by methanol-inducible alcohol oxidase (AOX) promoters. Recently this system has become an important source of recombinant G protein-coupled receptors (GPCRs) for structural biology and drug discovery. The influence of diverse culture parameters (such as pH, dissolved oxygen concen...

Hybrid bioreactor having both suspended-growth and attached-growth bacteria is found a novel and excellent bioreactor system for treating the municipal wastewater containing inhibitory substrates too. In this reactor a fraction of substrate is used by suspended biomass and the remaining by attached biomass resulting in the competition between the two growths for the substrate. The combination o...

Background The Allegro XRS 20 bioreactor system is a single-use bioreactor system suitable for applications ranging from general life sciences research to seed train operations as well as small scale production at the 2 to 25 literscale. It features a 3D rectangular biocontainer that is rocked across two independent axes perpendicular to one another. These features allow for significant improve...

As global fisheries decline, microbial single-cell protein (SCP) produced from brewery process water has been highlighted as a potential source of protein for sustainable animal feed. However, biotechnological investigation of SCP is difficult because of the natural variation and complexity of microbial ecology in wastewater bioreactors. In this study, we investigate microbial response across a...

Batch ethanol fermentations from sweet sorghum juice by Saccharomyces cerevisiae NP 01 were carried out in a 500 ml air-locked Erlenmeyer flask under very high gravity (VHG) and static conditions. The maximum ethanol production efficiency was obtained when 9 g l of yeast extract was supplemented to the juice. The ethanol concentration (P), productivity (Qp) and yield (Yp/s) were 120.24 ± 1.35 g...