Stem cells; therapeutics/trends; bone marrow. The use of autologous bone marrow cells in humans is celebrating ten years now, in an area where Brazilian cardiology has international recognition because of its pioneering initiative1. One decade ago the first cases were treated in patients with terminal chronic ischemic heart disease, which were published two years later2. The article by Vilas Bo...

Accumulated evidence suggests that in addition to hematopoietic stem cells (HSC), bone marrow (BM) also harbors endothelial stem cells (ESC), mesenchymal stem cells (MSC), multipotential adult progenitor cells (MAPC), pluripotent stem cells (PCS) as well as tissue committed stem cells (TCSC) recently identified by us. In this review we discuss the similarities and differences between these cell...

CSF-1 is a subclass of the colony-stimulating factors that specifically stimulates the growth of mononuclear phagocytes. We used the binding of 125I-CSF-1 at 0 degrees C by single cell suspensions from various murine tissues, in conjunction with radioautography, to determine the frequency of binding cells, their identity, and the number of binding sites per binding cell. For all tissues examine...

Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoietic stem cells for bone marrow reconstitution. Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoietic stem cells for bone marrow reconstitution. Separation and processing of UCB samples in large numbers for storage in cord blood banks ideally needs to be p...

The amount of prostaglandin E2 (PGE2) in the supernatant of human bone marrow cell cultures was measured by radioimmunoassay. Bone marrow cells were obtained from adult patients without hematological malignancies. Marrow mononuclear cells (BMMNC) (0.5 X 10(6)) and marrow nonadherent cells (BMNAC) (0.5 X 10(6)) were cultured at 37 degrees C in 5% CO2 in 0.5 ml RPMI 1640 supplemented with L-gluta...

Although estrogen is important in human skeletal homeostasis, the major target cell in bone is unknown. Estrogen receptors (ER) have been demonstrated in osteoblasts and bone marrow stromal cells, but their presence in osteoclasts remains controversial because completely pure preparations have not been available. We have examined expression of ER-alpha and ER-beta messenger RNA (mRNA) by RT-PCR...

II. Aetna considers autologous intra‐coronary mononuclear bone marrow cell transplantation or intra‐coronary administration of cardiosphere‐derived cells experimental and investigational for members with myocardial infarction and for all other cardiac diseases (e.g., chronic heart failure) because its effectiveness for these indications has not been established. Last Review 06/23/2016 Effective...