Echium amoenum Fisch. & Mey. (Boraginaceae) is a very popular medicinal plant that is used as a tonic, tranquillizer, diaphoretic, and a remedy for cough, sore throat and pneumonia in traditional medicine of Iran.
Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, we investigated callus culture of E. amoenum and the major secondary meta...