background and objective: due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission. materials and methods: to detect b. pertussis strains, we used two real-time pcr targeting is 481 and bp283 sequences and compared factors influencing culture and real-time pcr results. results: totally, 779 specimens were...

We describe the first clinical isolate of Bordetella petrii from a patient with mandibular osteomyelitis. The only previously documented isolation of B. petrii occurred after the initial culture of a single strain from an environmental source.

In many clinical microbiology laboratories, nucleic acid amplification tests such as PCR have become the routine methods for the diagnosis of pertussis. While PCR has greatly increased the ability of laboratories to detect Bordetella pertussis infections, it has also been associated with false-positive results that can, given the tendency of B. pertussis to cause outbreaks, result in unnecessar...

The effects of the CultureSwab (Difco Laboratories, Detroit, Mich.) and Culturette II (Marion Scientific, Div. Marion Laboratories, Inc., Kansas City, Mo.) transport systems on the viability of 95 clinically significant bacteria were studied. Organisms included staphylococci (8 isolates), streptococci (22 isolates), Haemophilus spp. (16 isolates), members of the family Neisseriaceae (14 isolate...