Cell-cell communication within the follicle involves many signaling molecules, and this process may be mediated by secretion and uptake of exosomes that contain several bioactive molecules including extra-cellular miRNAs. Follicular fluid and cells from individual follicles of cattle were grouped based on Brilliant Cresyl Blue (BCB) staining of the corresponding oocytes. Both Exoquick precipita...

AIM The aim of this study was to investigate in vitro the utility of biologically compatible, nontoxic and cell-specific targetable hydrogel nanoparticles (NPs), which have Coomassie® Brilliant Blue G dye (Sigma-Aldrich, MO, USA) covalently linked into their polyacrylamide matrix, as candidates for photothermal therapy (PTT) of cancer cells. MATERIALS & METHODS Hydrogel NPs with Coomassie Bri...

In order to obtain a compound stain to differentiate between mast and plasma cells, it was necessary to find dyes which provided a distinct colour contrast between the two types of cell. The aqueous acetic Alcian blue method (McManus and Mowry, 1960) stained mast cells, but also other tissue elements. Using Alcian blue at a pH less than 10 (Johnson and Helwig, 1963) the mast cells stained brill...

In previous studies, it was established that chitosan and its quaternized derivatives are potent enhancers of hydrophilic compounds absorption across intestinal epithelia. The aim of this study was to evaluate the application of a new quaternized chitosan, triethyl chitosan (TEC), in pharmaceutical approaches. TEC was synthesized by a one step process via a 2(2) factorial design to optimize the...

Efficiency of white-rot fungi-Stereum ostrea (S. ostrea) as a test culture and Phanerochaete chrysosporium (P. chrysosporium) as a reference culture in colour removal from a textile dye, Remazol brilliant blue-19 (RBB-19) in medium was compared in this study. S. ostrea was more efficient than P. chrysosporium in decoloration process. Different parameters pH, temperature, sources of carbon and n...

1. An accurate quantitative method of measuring the penetration of dye into the living cell is described. 2. Cresyl blue is unable to penetrate rapidly unless the pH outside the cell is decidedly greater than that inside. The rate of penetration increases with increasing pH. 3. Around pH 9 penetration of the dye is rapid while the reverse is true of exosmosis. At low pH values (5.9) exosmosis i...