In the present study, the accumulation of apolipoproteins (apo) A-I, B, and E in culture medium was measured after 0, 3, 6, 12, and 24 h of incubation with 150 microM docosahexaenoic acid complexed to 75 microM bovine serum albumin (BSA-22:6), either in the presence or absence of 50 micrograms/ml cholesterol and 4 micrograms/ml 25-hydroxycholesterol (C/25-OH). HepG2 cells incubated with BSA + C...

The reaction between reducing sugars and protein free amines, known as the Maillar reaction results in the formation of advanced glycation endproducts (AGEs). AGE modification changes the structure of proteins to amyloid cross-beta structure. These protein structures can activate receptors known as RAGE on glial cells (microglia and astrocytes), and induce the expression of inducible nitric oxi...

Terahertz spectroscopy was used to study the absorption of bovine serum albumin (BSA) in water. The Diamond Light Source operating in a low alpha mode generated coherent synchrotron radiation that covered a useable spectral bandwidth of 0.3-3.3 THz (10-110 cm(-1)). As the BSA concentration was raised, there was a nonlinear change in absorption inconsistent with Beer's law. At low BSA concentrat...

Optimal conditions were determined for photophosphorylation and reduction in mature chloroplasts from Phaseolus vulgaris var. Red Kidney. Bovine serum albumin (BSA) at 1 mg/ml and various sulfhydryl reagents (0.1-0.5 mm) greatly enhanced cyclic and noncyclic phosphorylation, but had little effect on photoreduction. BSA and reduced glutathione also stimulated cyclic phosphorylation in spinach ch...

A novel molecularly imprinted optosensing material based on multi-walled carbon nanotube-quantum dots (MWCNT-QDs) has been designed and synthesized for its high selectivity, sensitivity and specificity in the recognition of a target protein bovine serum albumin (BSA). Molecularly imprinted polymer coated MWCNT-QDs using BSA as the template (BMIP-coated MWCNT-QDs) exhibits a fast mass-transfer s...

Current work in the Martin group has focused on utilizing single conical nanopores prepared by the track-etch method [1] to detect proteins via the resistive pulse sensing technique by recording current-time (i-t) traces through a single pore. When an analyte passes through the pore, translocation events are observed in the i-t trace. In this study, we recorded translocation events for an indiv...

We reported previously that yeast-derived whole glucan particles (WGPs), with or without conjugation to BSA, used as a vaccine protected against systemic aspergillosis in mice. Here, we examined their utility as a potential vaccine against coccidioidomycosis. WGPs were prepared from Saccharomyces cerevisiae; conjugation with BSA (WGP-BSA) was done using 1-cyano-4-dimethylaminopyridinium tetrafl...

e widespread availability of minimally invasive hemodynamic monitors encourages evaluation of cardiac output (CO) and stroke volume (SV) in real time, and they are integral to goal-directed therapy, algorithmbased treatment plans. Patients with extremes of body surface area (BSA) are at risk of having their indices erroneously evaluated despite adequate non-indexed values. We evaluated the use ...