The aim of this work was to study the influence of vitamin E (2mM) on capacitation and acrosome reaction of crossbred cattle bull spermatozoa. Fresh semen suspended in TALP medium (pH 7.4) was divided into four equal fractions. All fractions; control (containing TALP + spermatozoa) and the experimental [treated / untreated with ferrous ascorbate (FeAA – 150 μM FeSO4 : 750 μM ascorbic acid); sup...

Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of chromatin in condensed sperm, we developed a novel combined heparin-reduced gl...

Introduction In addition to the requirements for healthy well-managed cattle and the sound application of synchronizing drugs, many other factors can also play a role in determining the success of an AI – estrous synchronization program. Considering the economic investment in semen and drugs, the success of such a program must be judged on the basis of pregnancy rate to the first artificial ins...

In addition to the requirements for healthy well-managed cattle and the sound application of synchronizing drugs, many other factors can also play a role in determining the success of an AI–estrous synchronization program. Considering the economic investment in semen and drugs, the success of such a program must be judged on the basis of pregnancy rate to the first artificial insemination servi...

Galectin-3, a member of the beta-galactoside-binding protein family, has been implicated in mammalian sperm maturation. We examined galectin-3 expression in the testis and epididymis of sexually mature and immature bulls. Western blot analysis showed varying levels of galectin-3 in the bull testis and epididymis, and galectin-3 immunoreactivity was higher in the mature testis and epididymis tha...

Abstract Flow cytometry has been used as a reliable and objective tool for bull sperm quality assessments identification of sub-fertility. Our was to determine if breed differences exist in using flow when evaluating semen from yearling Angus Charolais bulls that passed breeding soundness exam (BSE) evaluation thresholds. Ejaculates were collected via electroejaculation on one three consecutive...

The present study was conducted to analyze the fresh and post-thaw semen quality fertility from native bulls of Red Chittagong Cattle (RCC), BLRI Breed 1 (BCB1), Munshiganj Bangladesh. One hundred seventy-two ejaculates were collected by artificial vagina set analysis performed using Computer Assisted Sperm Analyzer (CASA) at Bangladesh Livestock Research Institute. Commercial extender (AndroMe...

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times o...