In order to optimize callus induction in Pistacia atlantica Desf. subsp. kurdica Rech. f., the effect of different plant growth regulator (PGR) combinations were studied. Inflorescence axis explants were cultured on WPM medium supplemented with 1, 2 and 3 mg/L of TDZ or 1, 2 and 3 mg/L of BA, in combination with 1mg/l different auxins: 2, 4-D,  NAA  and IBA . Callus initiation was observed in a...

The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the system from aspects genotype, medium components stress (PEG6000, mannitol, salt low phosphorus). results showed that GS07 had highest induction rate (95.2%). Orthogonal test analysis best combination in was A2B3C1D3(2), namely, concentration 2, 4-dichlorophenoxy acetic...

Different concentrations and combinations of hormones were used in MS medium to observe callus induction and then plant regeneration using leaf as explant. The rate of callus formation varied in different treatments used. The highest amount of callus (80.67%) was produced on MS medium containing 3.0 mg/l NAA from leaf after 20 days. The highest fresh weight of callus was obtained from 2.0 mg/l ...

An efficient protocol for hairy root induction in Berberis aristata DC. was established using two different strains of Agrobacterium rhizogenes, MTCC 532 and 2364 from IMTECH (Institute of Microbial Technology), Chandigarh, India. The strain 532 was more effective than strain 2364 in hairy root induction and in vitro grown callus (61.11 ± 1.60 % transformation frequency) was found to be suitabl...

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

koelreuteria elegans, popularly known as “flame gold” is an ornamental tree. in vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified ms medium. the highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-d from leaf segments. calli transferred in 1.5 mg/l bap resulted in efficient shoot regeneration...

The present study was conducted to investigate the effects of different concentrations and combinations of plant growth regulators (PGRs) on callus induction of Tecomella undulata (Sm) Seem. The leaf segments were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins such as NAA, 2,4-D, IAA and cytokikinins like BAP and KIN alone a...