نتایج جستجو برای: calyculin a

تعداد نتایج: 13431803  

Journal: :Integrative biology : quantitative biosciences from nano to macro 2017
Sophie Chagnon-Lessard Hubert Jean-Ruel Michel Godin Andrew E Pelling

The strain-induced reorientation response of cyclically stretched cells has been well characterized in uniform strain fields. In the present study, we comprehensively analyse the behaviour of human fibroblasts subjected to a highly non-uniform strain field within a polymethylsiloxane microdevice. Our results indicate that the strain gradient amplitude and direction regulate cell reorientation t...

Journal: :Endocrine 1999
M C Arufe G J Beckett R Durán M Alfonso

We have studied the effect of two protein phosphatase inhibitors on thyrotropin (TSH)-stimulated triiodothyronine (T3) production by sheep thyroid cells grown in primary culture. Incubation of sheep thyrocytes with okadaic acid (OA) and calyculin-A (CL-A), two potent inhibitors of type 1 (PP1) and type 2A (PP2A) protein phosphatases, resulted in an increase of TSH-stimulated T3 production. This...

Journal: :The Journal of pharmacology and experimental therapeutics 2008
Kun Don Yi Zu Yun Cai Douglas F Covey James W Simpkins

The mechanism of estrogen-mediated neuroprotection is not yet clear. Estrogens have a variety of modes of action, including transducing signaling events such as activation and/or suppression of the mitogen-activated protein kinase (MAPK) pathway. We have previously shown protein phosphatases to be involved in 17beta-estradiol-mediated neuroprotection. In the present study, we assessed the role ...

2014
April Reynolds Elizabeth A. Doggett Steve M. Riddle Connie S. Lebakken R. Jeremy Nichols

Missense mutations in the Leucine-Rich Repeat protein Kinase 2 (LRRK2) gene are the most common genetic predisposition to develop Parkinson's disease (PD) (Farrer et al., 2005; Skipper et al., 2005; Di Fonzo et al., 2006; Healy et al., 2008; Paisan-Ruiz et al., 2008; Lesage et al., 2010). LRRK2 is a large multi-domain phosphoprotein with a GTPase domain and a serine/threonine protein kinase dom...

2012
Stephen J. Fuller Liam J. McGuffin Andrew K. Marshall Alejandro Giraldo Sampsa Pikkarainen Angela Clerk Peter H. Sugden

The canonical pathway of regulation of the GCK (germinal centre kinase) III subgroup member, MST3 (mammalian Sterile20-related kinase 3), involves a caspase-mediated cleavage between N-terminal catalytic and C-terminal regulatory domains with possible concurrent autophosphorylation of the activation loop MST3(Thr(178)), induction of serine/threonine protein kinase activity and nuclear localizat...

2002
Tracy L. Davis Friederike Buerger Anne E. Cress

We have reported previously the existence of an Mr 70,000 form of the 6 integrin called 6p in a variety of human epithelial cell lines. Four different experimental conditions were used to examine the regulation of 6 and 6p integrin. The production of the 6 integrin was decreased by 45% using a protein translation inhibitor (2.25 M puromycin), whereas production of the 6p variant was unaffected....

Journal: :Neuron 1995
Kazutoshi Nakazawa Sumiko Mikawa Tsutomu Hashikawa Masao Ito

We generated a polyclonal antibody, 12P3, specifically recognizing rat AMPA-type glutamate receptor (GluR) subunits phosphorylated at Ser-696 of GluR2 or at the homologous sites in GluR1, GluR3, and GluR4. Using 12P3, we demonstrate that a brief exposure of a rat cerebellar slice to AMPA leads to transient phosphorylation of the GluR subunits in Purkinje cell dendrites. Persistent phosphorylati...

Journal: :Molecular cancer therapeutics 2009
Jun Mi Elzbieta Bolesta David L Brautigan James M Larner

In response to ionizing radiation, p53 plays a critical role in regulating DNA repair and apoptosis. Among multiple phosphorylation sites, evidence suggests that Ser46 promotes apoptotic cell death through mitochondrial outer membrane permeabilization (MOMP) and subsequent activation of the caspase 7-PARP pathway. Therefore, we investigated which phosphatase regulates Ser46 after ionizing radia...

Journal: :The Biochemical journal 1995
Y Yano M Sakon J Kambayashi T Kawasaki T Senda K Tanaka F Yamada N Shibata

Okadaic acid (OA) and calyculin A (CLA), which are potent and specific inhibitors of serine/threonine protein phosphatases type 1 and 2A, have been shown to induce drastic changes in platelet morphology. The aim of this study was to analyse the molecular mechanisms of OA- or CLA-induced cytoskeletal reorganization, with a specific focus on microtubules and actin filaments. Confocal fluorescence...

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