Carbapenem resistance in Enterobacteriaceae isolates has been reported from Turkey and is most often mediated by OXA-48 type carbapenemases. We report the identification and characterization of four carbapenem-resistant isolates (three Klebsiella pneumoniae and one Escherichia coli) among 515 clinical Enterobacteriaceae isolates collected during a 7-month study period in Ankara, Turkey. The fou...

We report on the clinical characteristics, antimicrobial therapy, and outcomes of 20 critically ill patients with severe OXA-48like infections. Carbapenem-based therapy demonstrated improved survival (odds ratio = 5.0) as compared with non-carbapenem therapy. Risk factors for mortality included Acute Physiology and Chronic Health Evaluation III score and length of stay, highlighting the signifi...

Multidrug-resistant and difficult-to-treat Acinetobacter baumannii may be responsible for nosocomial infections. The production of carbapenem-hydrolyzing class D β-lactamases (CHDLs) and extended-spectrum β-lactamase (ESBLs) of the GES type possessing a carbapenemase activity has been increasingly reported worldwide in A. baumannii. The aim of this study was to analyze the resistance mechanisms...

β-Lactamases threaten the clinical use of carbapenems, which are considered antibiotics of last resort. The classical mechanism of serine carbapenemase catalysis proceeds through hydrolysis of an acyl-enzyme intermediate. We show that class D β-lactamases also degrade clinically used 1β-methyl-substituted carbapenems through the unprecedented formation of a carbapenem-derived β-lactone. β-Lacto...

OBJECTIVE To report trends of extended spectrum beta lactamase (ESBL), multidrug resistant (MDR) ESBL and emergence of carbapenem resistant ESBL producing isolates of K. pneumoniae from Pakistan. METHODS Retrospective analysis of laboratory data was conducted (2002-2007). K. pneumoniae were isolated and identified from clinical samples using standard microbiological techniques. Antimicrobial ...

This bacterium has the propensity to acquire resistance rapidly to various classes of antimicrobial agents and so shows high rates of resistance to multiple antimicrobial agents [1, 2]. There is ongoing controversy as to whether carbapenem resistance results in an increased risk of mortality in patients infected with A. baumannii. The emergence of carbapenem-resistant A. baumannii is a signific...

development of antibiotic resistance in intensive care units (icus) is a worldwide problem. the purpose of this study was to evaluate the effect of an antibiotic stewardship program (asp) by carbapenems restriction on gram-negative antimicrobial resistance in icu. the study was designed in a 21 bedded general icu of a teaching hospital with two wings (one and two) in tehran, iran. carbapenem pr...

introduction: pseudomonas aeruginosa is a serious challenge for antimicrobial therapy, due to chromosomal mutations or intrinsic resistance to various antimicrobial agents, such as metallo-β-lactams (mbl). this study aimed to investigate the prevalence of β-lactamases encoding genes among p. aeruginosa strains isolated from intensive care unit (icu) patients by phenotypic and multiplex pcr meth...

background :    acinetobacter baumannii has become one of the most important causes of nosocomial infections in recent years. the organism seems to be resistant to most classes of antibiotics. carbapenems are considered the most effective drugs for treatment of these infections. however, increasing emergence of carbapenem resistant a. baumannii has become a major healthcare problem.   methods :...

Four isolates of Klebsiella pneumoniae obtained from patients at a Maryland medical centre exhibited reduced susceptibility to carbapenems and were found to produce the novel, class A, plasmid-mediated, carbapenem-hydrolysing enzyme, KPC-2. This enzyme has 99% identity with the plasmid-mediated, carbapenem-hydrolysing enzyme KPC-1, reported previously in a North Carolina K. pneumoniae isolate. ...