The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Upstream of the predicted start codon of katA is a sequence that closely matches ...

Catalase (EC 1.11.1.6) oxidizes ethanol to acetaldehyde within the brain and variations in catalase activity may underlie some consequences of ethanol consumption. The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings. In the first series of studies, levels of catalase were compare...

A catalase gene, ohktA, from an alkali- and halo-tolerant bacterium, Halomonas sp. SK1, on the pKK223-3, was expressed in the catalase-lacking Escherichia coli strain UM2. Highly purified catalase showing a single band on SDS-PAGE was obtained by two liquid chromatography steps on DEAE-Toyopear1 and Chelating-Sepharose Fast Flow. The enzyme, oHktA, shows high catalase activity with a pH optimum...

Stern (1) has found that as much as 0.13 N ethyl hydrogen peroxide is required to give 50 per cent inhibition of the destruction of hydrogen peroxide by catalase. This is difficult to understand in view of the high affinities of catalase for ethyl hydrogen peroxide in both the primary and secondary compounds (~2 X 10” M) (2), in which all the catalase hematins are bound to ethyl hydrogen peroxi...

The endemic occurrence of favism in certain Mediterranean regions provided an investigative opportunity for testing in vivo the validity of claims as to the role of catalase in protecting human erythrocytes against peroxidative injury. Reduced activity of catalase was found in the erythrocytes of six boys who were deficient in erythrocytic glucose-6-phosphate dehydrogenase (G6PD) and who were s...

Immunoelectrophoretic analysis of 25-day-old newborn and adult rat livers verified the presence of a 6-arc pattern of catalase observed earlier for purified rat hepatic catalase and for liver homogenates. Application of the immunoelectrophoretic method to fetal and newborn liver homogenates revealed a gradual shift from predominantly anodic arcs present in the fetal and early newborn period to ...

An alkali- and halo-tolerant bacterium with high catalase activity was isolated and identified as a new species of the genus Halomonas. Its catalase (HktA) was simply purified by two steps of liquid chromatography. A 71.4% yield of the catalase was obtained with 97% purity on SDS-PAGE. The specific activity of HktA (57,900 U/mg protein) was two times higher than that of bovine liver catalase. T...

INTRODUCTION A decrease in the liver catalase activity in tumor-bearing rats was reported by Greenstein and collaborators in 1941 (1). Since the appear ance of this early report, the same authors have investigated the effects of a relatively large num ber of different tumors upon liver catalase activity in rats and mice (2-5). These studies have shown that the decrease in activity of the liver ...

Recently it was reported that methanogens of the genus Methanobrevibacter exhibit catalase activity. This was surprising, since Methanobrevibacter species belong to the order Methanobacteriales, which are known not to contain cytochromes and to lack the ability to synthesize heme. We report here that Methanobrevibacter arboriphilus strains AZ and DH1 contained catalase activity only when the gr...

Type2 diabetes formerly known as non insulin dependent diabetes is the most common form of hyperglycemia, insulin resistance and relative insulin deficiency. The type 2 diabetes is caused due to metabolical disorder. Oxidative stress, through production of reactive oxygen species (ROS) to develop insulin resistance, beta cell dysfunction, impaired glucose tolerance there by impairing antioxidan...