background and aims: herpes simplex virus type 1 (hsv1) remains a potentially serious health problem world wide. all infected people, including asymptomatic ones, are potential sources for virus transmission. virus envelope contains at least 13 glycoproteins, which glycoprotein d is the major target of immune responses. the aim of this study was development of a specific method that is a more r...

background: cross antigenicity is the major problem in developing a reliable tool for immunodiagnosis and immunoprophylaxis of parasitic diseases. mixed infection due to different types of gastrointestinal parasites is more common than single species infection under field condition. methods: the present study was undertaken to detect antigenic cross-reactivity among haemonchus contortus, oesoph...

BACKGROUND An enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFA) were evaluated for serological diagnosis of human strongyloidiasis. METHODS Serum specimens obtained from 46 individuals infected with Strongyloides stercoralis, 37 healthy persons and 381 persons with other parasitic infections were tested using an IgG-ELISA that used crude antigen of S. sterc...

The immunostimulatory effects of methanolic extract from Pulicaria crispa were investigated in mice before and after infection with Schistosoma mansoni. Mice were subjected for daily intra-peritoneal injection by the extract (33 ng/mouse) for 10 successive days followed by infecting every mouse with 100 S. mansoni cercariae. Treatment with the extract induced significant increase (p < 0.05) in ...

objective(s) leishmania can lead to a broad spectrum of diseases, collectively known as leishmaniasis. the a2 gene/ protein family could be one of the most eligible candidate factors of virulence in visceral leishmaniasis (vl). the previous results confirmed that in leishmania infantum, several a2 proteins are abundantly expressed by the amastigote, but not the promastigote stage. as there are ...

169 Summary: Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). After infection with BLV there is no detectable viremia but there is a strong and persistent humoral immune response to structural proteins, essentially the gp51 envelope glycoprotein and the major core protein p24. Polymerase chain reaction (PCR) with primers used to amplify part of env gene, aga...

The ability to conditionally direct antibodies is a potentially powerful application for Synthetic Biology in Medicine. Here we show that control of antibody binding through site-specific, chemical phosphorylation of a recognition domain creates a 'gated' antibody (Ab). This displays a crude Boolean logic where binding is induced in an enzyme-AND-antigen dependent manner. This 'AND-Ab' is there...

The antigen involved in the glomerulonephritis associated with antibodies to glomerular basement membrane (GBM) was purified from human GBM digested with highly purified clostridial collagenase. The purified nonreduced sample contained two components with closely similar mobilities on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. After reduction they moved as one, nonantigenic, com...

Hybridomas producing monoclonal antibodies (MAbs) directed against P. vivax erythrocytic stage antigens were isolated. Here, we describe the production of six MAbs. Culture supernatant from growing hybridomas was tested for their reactivity with P. vivax erythrocytic stages by ELISA against P. vivax crude (PvC) antigen and by immunofluorescence assay on P. vivax infected erythrocyte smears. P. ...

A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot...