psinR is a clathrin-coated vesicle (CCV) enriched 70-kD protein that binds to phosphatidylinositol-4-phosphate, clathrin, and the gamma appendage domain of the adaptor protein complex 1 (AP1). In cells, its distribution overlaps with the perinuclear pool of clathrin and AP1 adaptors. Overexpression disrupts the CCV-dependent trafficking of cathepsin D from the trans-Golgi network to lysosomes a...

Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation of C. burnetii has paved the way for the application of a range of genetic techniques to address ...

Despite recent advances in mass spectrometry, proteomic characterization of transport vesicles remains challenging. Here, we describe a multivariate proteomics approach to analyzing clathrin-coated vesicles (CCVs) from HeLa cells. siRNA knockdown of coat components and different fractionation protocols were used to obtain modified coated vesicle-enriched fractions, which were compared by stable...

Coxiella burnetii is an intracellular pathogen that replicates in a lysosome-derived vacuole. The molecular mechanisms used by this bacterium to create a pathogen-occupied vacuole remain largely unknown. Here, we conducted a visual screen on an arrayed library of C. burnetii NMII transposon insertion mutants to identify genes required for biogenesis of a mature Coxiella-containing vacuole (CCV)...

Functional imaging studies have delineated a "minimal network for overt speech production", encompassing mesiofrontal structures (supplementary motor area, anterior cingulate gyrus), bilateral pre- and postcentral convolutions, extending rostrally into posterior parts of the inferior frontal gyrus (IFG) of the language-dominant hemisphere, left anterior insula as well as bilateral components of...

To the Editor The publication of the article of Enriquez et al 1 coincided with the final steps of our study on the expression of CDX2 in normal and neoplastic follicular-derived cells of the human thyroid gland. Enriquez et al 1 evaluated CDX2 expression in 11 cases of columnar cell variant (CCV) of papillary thyroid carcinoma (PTC) and in thyroid tissue microarrays (TMAs) composed of normal t...

Content-Based Image Retrieval (CBIR) uses the visual contents of an image such as color, shape, texture, and spatial layout to represent and index the image. Active research in CBIR is geared towards the development of methodologies for analyzing, interpreting cataloging and indexing image databases. In addition to their development, efforts are also being made to evaluate the performance of im...

Mechanisms of clathrindependent endocytosis Although clathrin-mediated internalization has been investigated for many years, new technologies keep providing us with novel insights into its underlying mechanisms, with an unprecedented scale down to molecular details regarding the structure and dynamics of the proteins involved. The plasticity of clathrin-mediated internalization was well illustr...

This dissertation presents two statistical methodologies developed on multi-order Markov models. First, we introduce an alignment-free sequence comparison method, which represents a sequence using a multi-order transition matrix (MTM). The MTM contains information of multi-order dependencies and provides a comprehensive representation of the heterogeneous composition within a sequence. Based on...