INTRODUCTION This study aimed to examine the prevalence of opportunistic infections in HIV-infected patients in Bahrain and its relation to absolute CD4 count, CD4%, and CD4/CD8 ratio. METHODOLOGY This retrospective cohort study used laboratory records (January 2009 - May 2013) from a major hospital in Bahrain. Opportunistic infections (OIs); absolute CD4 counts, CD4%, and CD4/CD8 ratio were ...

Background: Erythroderma is an inflammatory disorder. It has various differential diagnoses, among which one of the most important is mycosis fungoides. Erythroderma itself can be a challenging disorder. Diagnosis of a mycosis fungoides patient presenting with erythroderma specially requires a careful assessment of the peripheral blood. Studies such as CD markers can lead to a more accurate dia...

Thalidomide is well documented as being an effective drug in the treatment of erythema nodosum leprosum (ENL). The mechanism of action of thalidomide in ENL as well as the pathogenesis of ENL are yet to be fully determined. Lepromatous leprosy patients experiencing ENL have been reported to have an increase in the ratio of CD4+ to CD8+ cells in their blood and ENL skin lesions. Thalidomide has ...

In order to characterize the cellular composition of cerebrospinal fluid (CSF) in a healthy state and in the setting of chronic pleocytosis associated with HIV-1 (HIV) infection, multi-parameter flow cytometry was used to identify and quantitate cellular phenotypes in CSF derived from HIV-uninfected healthy controls and HIV-infected subjects across a spectrum of disease and treatment. CD4+ T ce...

We investigated the relationship between peripheral blood T lymphocyte subsets and hepatitis C virus (HCV) RNA levels in patients with hepatitis C. Samples from 69 chronic hepatitis C (CHC) patients and 20 healthy controls were analyzed using quantitative polymerase chain reaction (PCR) to detect HCV RNA and flow cytometry to determine the expression levels of CD3, CD4, and CD8 in lymphocytes. ...

Patients with hemophilia A without human immunodeficiency virus type 1 (HIV-1) infection have lower CD4+ counts and CD4+/CD8+ ratios than controls. This is usually interpreted as a therapy-induced immunodeficiency. Our data re-examine the effect of therapy on peripheral blood mononuclear cell immunophenotypic subpopulations in all congenital clotting disorders. Since late 1985 we have prospecti...

In follicular lymphoma, nonmalignant immune cells are important. Follicular lymphoma depends on CD4+ cells, but CD8+ cells counteract it. We hypothesized that the presence of follicular lymphoma is associated with higher CD4+ than CD8+ cell numbers in the tumor microenvironment but not in the immune system. Using flow cytometry, pre-treatment and follow-up CD4/CD8 ratios were estimated in the b...

IntroductionThis study aimed to analyse cervical lymphocytic populations in HIV+ and HIV− patients correlate different lesions with HIV viral load presence of high-risk HPV types.Material methodsA total 132 histological specimens from 40 72 were evaluated for CD4+ CD8+ T cell distribution, types, peripheral blood CD4+/CD8+ ratio.ResultsHigh-grade squamous intraepithelial (HSIL) carcinoma (SCC) ...

The organotypic tumor slice culture model resembles the in vivo situation of tumors. It may serve as a good ex platform for response to anti-tumor therapies. We aimed evaluate pancreatic ductal adenocarcinoma (PDA) cells and helper, cytotoxic, regulatory T gemcitabine using human PDA model. tissues were obtained from patients who operated on resection. A vibratome sliced tumors, slices cultured...