Cytotox ic T-cel ls were derived from the per i toneal cavity of a C 5 7 B L / 6 mouse immunized with B A L B / c sarcoma Meth A and from the sp leens of B A L B / c x C 5 7 B L / 6 F1 (hereaf ter cal led CB6F1) mice immunized with B A L B / c leukemia R L ~ I . The cel ls were cu l tured in inter leukin 2 and c loned by limiting di lut ion, and their speci f ic i ty was determined by d i rect ...

Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects. In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally re...

The molecular co-chaperone CDC37 is over-expressed in hepatocellular carcinoma (HCC) cells, where it functions with HSP90 to regulate the activity of protein kinases in multiple oncogenic signaling pathways that contribute towards hepatocarcinogenesis. Disruption of these signaling pathways via inhibition of HSP90/CDC37 interaction is therefore a rational therapeutic approach. We evaluated the ...

Idiopathic hypereosinophilic syndrome (HES) and chronic eosinophilic leukemia (CEL) comprise a spectrum of indolent to aggressive diseases characterized by unexplained, persistent hypereosinophilia. These disorders have eluded a unique molecular explanation, and therapy has primarily been oriented toward palliation of symptoms related to organ involvement. Recent reports indicate that HES and C...

Aims. We present an analysis of physical conditions in planetary nebulae (PNe) in terms of collisionally-excited line (CEL) and optical-recombination line (ORL) profiles. We aim to investigate whether line profiles could be used to study the long-standing CEL/ORL abundance-discrepancy problem in nebular astrophysics. Methods. Using 1D photoionization models and their assumed velocity fields, we...

BACKGROUND Single nucleotide polymorphism (SNP) markers are the method of choice for genetic analyses including diversity and quantitative trait loci (QTL) studies. Marker validation is essential for QTL studies, but the cost and workload are considerable when large numbers of markers need to be verified. Marker systems with low development costs would be most suitable for this task. RESULTS ...

In in tes t ina l mucosa of fed adult rats, cytochrome P-450 content and ac t i v i t y of benzpyrene hydroxyl ase and p-n i t roaniso le O-demethylase are highest in the upper small in test ine and progressively decrease toward the terminal ileum. Among the mucosal cell populations, mature v i l l ous t i p cel ls contain 6 to I0 times more cytochrome P-450 and drug-metabolizing ac t i v i t y...

The midgut environment of anopheline mosquitoes plays an important role in the development of the malaria parasite. Using genetic manipulation of anopheline mosquitoes to change the environment in the mosquito midgut may inhibit development of the malaria parasite, thus blocking malaria transmission. Here we generate transgenic Anopheles stephensi mosquitoes that express the C-type lectin CEL-I...

Various streptomyces strains [Streptomyces lividans 66, Streptomyces vinaceus, and Strepotmyces coelicolor A3 (2)] acquired the ability to utilize crystalline cellulose (Avicel) after transformation with a multicopy vector containing the cel-1 gene from Streptomyces reticuli. The expression level in these hosts was two to three times lower than in S. reticuli, indicating the absence of positive...

In chronic myelogenous (CML) and chronic eosinophilic leukemia (CEL), neoplastic cells spread via the circulation into various extramedullary organs. As E- and P-selectin constitute the starting point for the leucocyte adhesion/invasion cascade, and CEL and CML cells share many properties with normal granulocytes, we investigated the role of these selectins in CEL and CML cell expansion and org...