T-cell adhesion/costimulatory molecules and their cognate receptors on target cells play a major role in T-cell receptor (TCR)–mediated activities. Here, we compared the involvement of CD103 and LFA-1, and their respective ligands, in the maturation of the cytotoxic immune synapse (cIS) and in the activation of CTL effector functions. Our results indicate that cytotoxicity toward cancer cells a...

The relationship between membrane permeability, changes in ultrastructure, and inactivation in Escherichia coli strain K-12TG1 cells subjected to high hydrostatic pressure treatment at room and subzero temperatures was studied. Propidium iodide staining performed before and after pressure treatment made it possible to distinguish between reversible and irreversible pressure-mediated cell membra...

The behavior of the cancer cell is determined in part by peculiarities of its external surface. For example, the cancer cell is unable adequately to bind calcium (7), and, as a consequence, cancer cells do not adhere to one another tightly (5, 10) but tend to separate, migrate (8), and to invade the surrounding tissues. Thus, the invasive or malignant features of cancer may be attributed largel...

Synaptic ultrastructure is critical to many basic hypotheses about synaptic transmission. Various aspects of synaptic ultrastructure have also been implicated in the mechanisms of short-term plasticity. These forms of plasticity can greatly affect synaptic strength during ongoing activity. We review the evidence for how synaptic ultrastructure may contribute to facilitation, depletion, saturati...

Mouse embryonic stem cells can differentiate in vitro and form embryoid bodies containing structures and cells that differ among all three germ layers. The fine structure of mouse ES cells and that of their respectively differentiated EBs were analyzed by transmission electron microscopy. We observed that mES cells remained undifferentiated and expressed undifferentiated markers such Oct4, Nano...

Wood is complex in its chemical composition that has an important influence on behavior and mechanical strength. The complexity reflected the ultrastructure of wood cell wall. In particular, concentration main components (cellulose, hemicelluloses lignin) changes depending many factors such as different type or parts wood, varies wall layers. From ultrastructural standpoint, we describe current...

The first-generation precursors producing adult-born neurons in the crayfish (Procambarus clarkii) brain reside in a specialized niche located on the ventral surface of the brain. In the present work, we have explored the organization and ultrastructure of this neurogenic niche, using light-level, confocal and electron microscopic approaches. Our goals were to define characteristics of the nich...

The antibacterial action of antibody (normal and hyperimmune), complement, and lysozyme has been studied by correlating the ultrastructural and biochemical changes that they cause in smooth Escherichia coli. Both normal and hyperimmune antibody, in the absence of lysozyme, produced complement-dependent release, into the suspending medium, of 63 to 72% of the (32)P-labeled phospholipid and 74 to...

T-cell adhesion/costimulatory molecules and their cognate receptors on target cells play a major role in T-cell receptor (TCR)-mediated activities. Here, we compared the involvement of CD103 and LFA-1, and their respective ligands, in the maturation of the cytotoxic immune synapse (cIS) and in the activation of CTL effector functions. Our results indicate that cytotoxicity toward cancer cells a...

Isoflurane can induce widespread cytotoxicity. We hypothesized that isoflurane induces apoptosis partly by causing excessive calcium release from the endoplasmic reticulum (ER) via direct activation of inositol 1,4,5-trisphosphate receptors (IP3R). Rat pheochromocytoma cells cultured for seven days with nerve growth factor were divided into four groups: control group (C), IP3R antagonist group ...