Preparative isopycnic density-gradient centrifugation is ordinarily done in high-speed swinging-bucket rotors such as the Spinco SW 39 and SW 25. In addition to requiring this special equipment, the technique is severely limited by the small number of samples and the low total-sample volume which may be used. In recent studies on methods for isolating small numbers of virus particles from tissu...

This report outlines the background to the development of an automated, serial, discrete centrifuge, reporting on the criteria considered essential in such an instrument. We established the criteria by examining the detailed logistics of centrifuge operation in a hospital laboratory. The mean sample load per run, using six centrifuges, was 13.6 samples, and the user-selectable cycle time ranged...

INTRODUCTION Centrifuge-induced artificial gravity (AG) with exercise is a promising comprehensive countermeasure against the physiological de-conditioning that results from exposure to weightlessness. However, body movements onboard a rotating centrifuge are affected by both the gravity gradient and Coriolis accelerations. The effect of centrifugation on squat exercise biomechanics was investi...

A major strategy for purifying and isolating different types of intracellular organelles is to separate them from each other based on differences in buoyant density. However, when cells are disrupted prior to centrifugation, proteins and organelles in this non-native environment often inappropriately stick to each other. Here we describe a method to separate organelles by density in intact, liv...

BACKGROUND A concentration of specimen is recommended for the effective recovery of Mycobacterium tuberculosis (MTB), but the bacteriological efficiency is not well evaluated. The present study evaluated the factors contributing to concentration efficiency of centrifugation and bead-based technique (TB-Beads; Microsens, UK) to recover MTB by using simple in vitro specimens. METHODS Four speci...

Laboratory investigations often require centrifugation of blood samples for various erythrocyte tests. Although there is a lack of data about the effect of centrifugation at various g force levels on erythrocyte rheological properties. We aimed to investigate the effect of a 10-minute centrifugation at 500, 1000 or 1500 g at 15°C of rat, dog, pig and human venous (K3-EDTA, 1.5 mg/ml) blood samp...

IPIT initial experiments on the fractionation of mitochondria into subparticulate constituents, it became apparent that for isolation of the particles, a method more satisfactory than the customary methods of differential centrifugation was needed. The subject of this report is a simple and rapid method for obtaining relatively uncontaminated preparations of particulate cell components by gradi...

Chlorella vulgaris proteins show high potential for food applications. The challenge of biorefinery is to choose the best processes coupling which can ensure integrity molecules and limit energy consumption. This study aimed at understanding impact biomass concentration physicochemical conditions during cell disruption, on whole process (bead milling (BM), centrifugation, microfiltration). scen...

The re-isolation of trace anlOunts of virus added to tissue hOInogenates has been taken as a Inodel probleIll in the developIllent of high-resolution Illethods for separating particles. FroIll a review of data on these particles, it was found that, when sediIllentation rates are plotted against their banding or buoyant densities, IllOst viI'uses fall in a "window" which appears to be free of ot...