A new modification of polyacrylamide gel electrophoresis (PAGE) was applied to cerebrospinal fluid proteins from patients with multiple sclerosis (MS). The same spinal fluids were also examined by a cytological technique. Over 90% of patients with clinically definite or early probable or latent MS showed abnormal PAGE patterns in the form of oligoclonal gammaglobulin bands. Reactive (atypical, ...

A 32-year-old woman presented with low pressure headache 3 days after delivery of her baby. An assessment of postdural puncture headache was made. This was initially treated with analgesia, caffeine, and fluids for the presumed cerebrospinal fluid (CSF) leak. The woman was readmitted two days after her hospital discharge with generalised seizures. A brain scan showed features of intracranial hy...

Background: Cerebrospinal fluid analysis is a very important tool in the diagnosis of bacterial meningitis in the new born. Bacterial meningitis is a potent cause of morbidity and mortality in the neonatal age group, with a very high incidence of neurological complications. Aim: To identify the bacterial pathogens isolated from the cerebrospinal fluids of neonates at the University of Port Harc...

Cerebrospinal fluid (CSF) is the body fluid closest to the pathology of multiple sclerosis (MS). For many candidate biomarkers CSF is the only fluid that can be investigated. Several factors need to be standardized when sampling CSF for biomarker research: time/volume of CSF collection, sample processing/storage, and the temporal relationship of sampling to clinical or MRI markers of disease ac...

We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-ric...

This article presents a case that highlights the importance of excluding underlying intracranial pathology in patient presenting with severe headache and positive xanthochromia. report demonstrated false-positive xanthochromia without subarachnoid haemorrhage (SAH) is possible acute bacterial meningitis when there combination traumatic lumbar puncture either hyperbilirubinaemia or raised cerebr...

EPTB comprises 10-15% of all TB cases in developing countries. Diagnosis from body fluids like pleural, peritoneal and cerebrospinal fluid (CSF) is challenging as these samples possess very few bacilli. : To determine the role adenosine deaminase (ADA) assay for reliable prediction different fluids, particularly low-resource areas with high disease prevalence. This prospective study was out a r...