نتایج جستجو برای: chelating assay
تعداد نتایج: 232461 فیلتر نتایج به سال:
The present work was concerned with the isolation and screening of tyrosinase enzyme producing marine actinobacteria from marine salterns soil. It was also focussed on testing the antioxidant and haemolytic activity of the isolated marine actinobacteria. A total of 17 actinobacterial isolates were isolated from salterns soil samples from Kothapattanam, Ongole, Andhra Pradesh. They were screened...
This study evaluated the effect of derriobtusone A, a flavonoid isolated from Lonchocarpus obtusus, on two important pathogenic bacteria, Staphylococcus aureus and Escherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scaven...
We have established a PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with primers specific to adhesin subunit gene. The minimum amount of H. pylori DNA that could be detected was 0.4 pg. Seventy-two antral biopsy specimens were taken from patients with gastritis and/or duodenal ulcer. H. pylori was found in 58.3%, 44.4% and 54.2% of patients according to the resu...
In vitro biological assessment of Homalium zeylanicum and isolation of lucidenic acid A triterpenoid
Homalium zeylanicum (Gardner) Benth. (Flacourtiaceae) is a medicinal plant useful in controlling rheumatism, inflammation and diabetes. The objective of this work evaluates in vitro antioxidant, antidiabetic, and antiinflammatory properties of hydroalcohol extract of bark of H. zeylanicum (HAHZ). It also describes isolation and structure determination of lucidenic acid A, which is the first rep...
The potency of Solenostemma argel (asclepiadaceae) as anti-inflammatory and antioxidant effects was investigated. Three solvent extract were used for extracting (polyphenolic, flavonoids and tannins) from the leaves of Solenostemma argel (S. argel). Three complementary antioxidant systems: (DPPH∙ free radical scavenging, Fe2+-chelating and iron reducing power) as well as the total phenolic, tot...
The present study reports the antioxidant and membrane protective activities of Calotropis procera aqueous root extract using several in vitro assays along with the determination of phenolic as well as flavonoid contents. Total phenol and flavonoid contents in extract were 15.67 ± 1.52 mg propyl gallate equivalent/g and 1.62 ± 0.05 mg quercetin equivalent/g, respectively. UV-visual spectroscopi...
The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating (FIC), and β -carotene bleaching (BCB) activity) of crude extract and total saponin fraction of ...
A novel class of platinum(IV) complexes of the type [Pt(Am)(R(COO)2)2], where Am is a chelating diamine or two monodentate am(m)ine ligands and R(COO)2 is a chelating dicarboxylato moiety, was synthesized. For this purpose, the reaction between the corresponding tetrahydroxidoplatinum(IV) precursors and various dicarboxylic acids, such as oxalic, malonic, 3-methylmalonic, and cyclobutanedicarbo...
We describe here a new microquantification method of l-phenylalanine concentration in an extract from a dried blood spot by using the diaphorase-resazurin system. To miniaturize the fluorometric enzymatic microplate assay for the diagnosis of phenylketonuria, an enzyme chip immobilized with His-tag fused phenylalanine dehydrogenase (PheDH) was developed. His-tag fused PheDH was immobilized on t...
Four known compounds have been isolated from the stems of Liriodendron tulipifera, and the structures of these pure constituents were determined using spectroscopic analysis. Isolated compounds were screened for free radical scavenging ability, metal chelating power assay and ferric reducing antioxidant power assay (FRAP). The anti-tyrosinase effects of L. tulipifera compounds were calculated t...
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