When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence. Luminol-dependent light emission from PMNL is linked to the myeloperoxidase (MPO)-H2O2 system. Light emission from a cell-free MPO-H2O2 system was found to be totally inhibited by human serum albumin (HSA), and since HSA is a large molecular protein that does not readily gai...

NADPH oxidation and cytochrome c reduction with and without lucigenin as well as NAD(P)H/lucigenin-dependent chemiluminescence of rat liver microsomes were studied. An increased rate of NADPH oxidation and cytochrome c reduction in the presence of lucigenin was related to one-electron lucigenin reduction by microsomal NADPH reductases. The apparent Michaelis constant values for lucigenin (Km ap...

Granulocytes and monocytes are known to phagocytose and kill numerous varieties of bacteria and parasites. This function is intimately associated with an early event called the "respiratory burst," which includes the generation of 02and H202 (1). These and other highly reactive molecular species can easily be detected in the presence of luminol or other readily oxidized substrates by the emissi...

The exact time profile of superoxide generation during anoxia-reoxygenation and ischemia reperfusion was assessed in the feline cerebral cortex in vivo using a chemiluminescence technique with a probe specific for superoxide, 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-ƒ¿]pyrazin 3-one (MCLA). MCLA solution was superfused on the cortex throughout the protocol, and MCLA chemiluminescence...

Because of controversies in the literature on nitric oxide (NO) production by plants, NO detection by the frequently used diaminofluorescein (DAF-2 and DAF-2DA) and by chemiluminescence were compared using the following systems of increasing complexity: (i) dissolved NO gas; (ii) the NO donor sodium nitroprusside (SNP); (iii) purified nitrate reductase (NR); and (iv) tobacco cell suspensions. L...

Plasma from hemodialysis patients evoked weak photon emissions (chemiluminescence) in a characteristic emission spectrum with a peak at 430 nm, attributed to attack by hydroxyl radicals generated from the iron-catalyzed breakdown of hydrogen peroxide (Fenton reaction), whereas plasma from normal healthy subjects showed a rather weak red chemiluminescence peak at around 680 nm, similar to that r...

dried aceton-chloroform extract of aerial parts of euphorbia spinidens bornm. ex prokh. endemic to iran, yielded two new triterpenoids, lup-20(29)-ene-33, 28 diol commonly known as betulin and (3β,23e)-cycloarta-23-ene-3,25-diol. the structures of the isolated compounds were elucidated by 13 c- and 1 h-nmr as well as 2d-nmr, ir and by the aid of mass fragmentation pattern. in phagocyte chemilum...

Replacing the peroxide bond of dioxetanone prevents chemiluminescence by making its thermolysis energetically unfavorable and without a singlet chemiexcitation pathway.

Surface plasmon spectroscopy (SPS) is commonly implemented as an analytical tool to characterize thin kinetic processes and binding reactions at interfaces.1 Due to the limited sensitivity of SPS for the detection of dilute analytes of low molecular mass, surface plasmon coupled fluorescence (SPCF) or, alternatively, surface plasmon coupled emission (SPCE) has been proposed as an alternative me...