1. A strain of neurovaccine virus was cultivated in a medium consisting of minced chick embryo suspended in Tyrode's solution. 2. The virus upon cultivation apparently lost none of its essential characteristics. 3. The culture virus can be preserved and stored for long periods of time. Furthermore, the preserved virus can be used to initiate fresh cultures.

BACKGROUND Ischemia is a pathophysiological condition due to blockade in blood supply to a specific tissue thus damaging the physiological activity of the tissue. Different in vivo models are presently available to study ischemia in heart and other tissues. However, no ex vivo ischemia model has been available to date for routine ischemia research and for faster screening of anti-ischemia drugs...

The fate map of the early neural plate and neural fold has been established at the cephalic level by using the quail-chick marker system (Le Douarin, 1969,1973). The experimental design comprised the replacement of definite territories belonging to the neural plate and neural folds in the chick embryo by their counterparts from quail embryos at the same developmental stage. This technique is re...

1. A strain of meningococci obtained directly from the spinal fluid of a patient has been propagated in serial passage in 10 to 12 day old chick embryos without change in its essential characteristics. 2. The chick embryo is susceptible to infection with the meningococcus, and, depending on its stage of development, reacts to the infection with more or less specific lesions. 3. In chick embryos...