Permeability of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel to polyatomic anions of known dimensions was studied in stably transfected Chinese hamster ovary cells by using the patch clamp technique. Biionic reversal potentials measured with external polyatomic anions gave the permeability ratio (P/P) sequence NO > Cl > HCO > formate > acetate. The same select...

The physicochemical structure of the mixing water used in concrete has a significant effect on physical and mechanical properties cementitious composites. studies magnetized (MW) FA/BFS-based composites are still their infancy. This study explores MW fresh hardened fly ash (FA)/blast furnace slag (BFS)-based A total 22 different mixture groups having FA/BFS (0, 5, 10, 15, 20, 25%) by weight cem...

The cataract which develops in the rat fed triparanol is accompanied by a marked increase in sodium and a more modest decrease in the potassium. Sodium is excreted as the lens clears and potassium returns to normal. Initially triparanol causes an increaese in permeability to cations. This appears before the lens becomes cataractous. Permeability returns to normal as the lens clears. In the cata...

A sensitive fluorometric assay for measuring permeability of liposomes containing bovine lens MIP26 has been developed and characterized. Lens membrane proteins were isolated and incorporated into artificial membranes composed of bovine brain phospholipids, sphingomyelin and the fluorescent probe, N-4-nitrobenzo-2-oxa-1,3 diazole phosphatidylethanolamine (NBD-PE). Quenching of the probe with co...

Data are presented which demonstrate acclimation of brown trout (Salmo trutta) to an acid medium. Sodium fluxes, transepithelial gill potentials and plasma electrolyte concentrations were measured in brown trout exposed to an acid medium of pH 6.0 for a period of 6 weeks. Sodium loss rates in acid exposed fish are reduced compared to normal fish, and sodium uptake in insensitive to external pH ...

Sweat test with Gibson Cooke (GC) method is the diagnostic gold standard for cystic fibrosis (CF). Recently, alternative methods have been introduced to simplify both the collection and analysis of sweat samples. Our aim was to compare sweat chloride values obtained by GC method with other sweat test methods in patients diagnosed with CF and whose CF diagnosis had been ruled out. We wanted to d...

Nanocomposites were synthesized from reactive thermally stable montmorillonite and aromatic-aliphatic polyamide obtained from 4-aminophenyl sulfone and sebacoyl chloride. Carbonyl chloride terminal chain ends were generated using 1% extra sebacoyl chloride that could interact chemically with the organoclay. The distribution of clay in the nanocomposites was investigated by XRD, SEM, and TEM. Me...

The present study was designed to observe the properties of swelling-activated chloride channel (ICl.swell) in mouse cardiac myocytes using patch clamp techniques. In whole-cell recordings, hypotonic solution activated a chloride current that exhibited outward rectification, weak voltage-dependent inactivation, and anion selectivity with permeability sequence of I- > Br- > Cl-. The current was ...