Five different protocols, viz. Andleeb et al. (2012), Shrivastava et al. (2012), Phenol chloroform DNA extraction method, Roboscreen Kit method and Phenol chloroform triton method, were tested for DNA extraction from skin samples of Leopard (Panthera pardus) preserved in formalin over a period of two to five months. Among these procedures, the Phenol chloroform triton method was proved to be th...

The question of pregnancy and the use of anesthetics is a most important one and has called forth numerous contributions and a large amount of speculation and controversy. The opinion seems to be gaining ground that chloroform is an unsatisfactory anesthetic because of danger during the time of anesthesia, and, perhaps, more because of the possibility of the so-called effects of "late chlorofor...

Trihalomethanes (chloroform, bromodichloromethane, chlorodibromomethane, and bromoform) are regulated organic contaminants in chlorinated drinking water. In female B6C3F1 mouse liver, the 4 trihalomethanes have demonstrated carcinogenic activity when administered by oral gavage; however, chloroform was not carcinogenic when administered in drinking water. Female B6C3F1 mice were administered th...

Chloroform is a known contaminant of chlorinated drinking water and of swimming pool water disinfected with chlorine or one of its derivatives. Few data exist regarding the importance of dermal and inhalation exposure routes to the chloroform body burden resulting from domestic and recreational use of chlorinated water. In our experimental study involving 11 male swimmers, we quantified the bod...

In spite of many investigations concerning the distribution and fate of anesthetic and lethal doses of chloroform in animal fluids, tissues, and organs,l there have been relatively few accurate quantitative determinations of the amounts of chloroform in various parts of the animal body following chloroform poisoning. The most reliable data are evidently those reported by Buckmaster and Gardner ...

To study the origin of chloroform in vegetables, a simple and sensitive method was established using a gas chromatograph equipped with an electron capture detector. The method includes solvent trapping of chloroform, and gas chromatographic separation with a detection system. The average concentrations in vegetables, tea leaves and frozen vegetables were 30.9, 62.9 and 15.7 μ/kg, respectively. ...