background: cholesterol oxidase (cho) has various clinical and industrial applications. recently, microbial cho have received a great attention for their wide usage in medicine. here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for cho production and biochemical characterizations of produced cho enzyme were described. finally, cho gene was cloned int...

Background MiRNAs are small non-coding RNAs involved in many biological functions such as cell proliferation and apoptosis (1), cell cycle (2), homeostasis (3) and cell metabolism (4). They are highly conserved between species. They are capable of regulating hundreds of genes in a post-transcriptional manner by translation repression and/or mRNA degradation (5). These characteristics make miRNA...

Bovine lactoferrin inhibits the clearance of remnant lipoproteins from the plasma and competes with the cell-surface binding of apolipoprotein (apo) E-enriched remnants. We established that lactoferrin inhibits remnant binding and uptake by interacting with both heparan sulfate proteoglycans (HSPG) and the low-density lipoprotein receptor-related protein (LRP). The binding of 125I-lactoferrin w...

MicroRNAs (miRNAs) have recently entered Chinese hamster ovary (CHO) cell culture technology, due to their severe impact on the regulation of cellular phenotypes. Applications of miRNAs that are envisioned range from biomarkers of favorable phenotypes to cell engineering targets. These applications, however, require a profound knowledge of miRNA sequences and their genomic organization, which e...

We have reported previously the isolation and characterization of a Chinese hamster ovary cell line, designated CHO-Chlr, which exhibits resistance to bifunctional nitrogen mustards while maintaining sensitivity to a range of other alkylating agents and chemotherapeutic drugs. This enhanced drug resistance is associated with a greater than 40-fold increase in the level of expression of an alpha...

Background: Breast cancer is the most common female malignancy and the leading cause of cancer mortality in women worldwide. The human epidermal growth factor receptor2 (HER2) is a transmembrane tyrosine kinase receptor that is usually overexpressed in human breast cancers. Stable cell lines heterogeneously overexpressing HER2 are highly required as in vitro models for breast cancer research. T...

The phosphorylation of H1 histone subtypes was studied in 3 Chinese hamster cell lines (CHO, V79, and CHW). Chromatographic resolution of H1 subtypes showed that all 3 cell lines contained 1 homologous (coeluting) H1 subtype (CHO-1, V79-1, and CHW-1) while V79 and CHW cells contained 2 additional H1 subtypes not found in CHO cells (V79-2,3 and CHW-2,3). N-Bromosuccinimide cleavage of 32P-labele...

Introduction Expression of recombinant antibodies in CHO cells is a state-of-the-art procedure in research and industry. Generation of cell lines producing high amounts of antibodies is one of the major tasks to increase process efficiency. The establishment of clones is often achieved by methotrexate (MTX)–mediated gene amplification in CHO-DG44 cells. Evaluation of MTX-mediated amplification ...

Sperm PLCζ (phospholipase Cζ) is a distinct phosphoinositide-specific PLC isoform that is proposed to be the physiological trigger of egg activation and embryo development at mammalian fertilization. Recombinant PLCζ has the ability to trigger Ca²⁺ oscillations when expressed in eggs, but it is not known how PLCζ activity is regulated in sperm or eggs. In the present study, we have transfected ...