PURPOSE Identification of new targeted therapies is critical to improving the survival rate of patients with osteosarcoma. The goal of this study is to identify kinase based potential therapeutic target in osteosarcomas. EXPERIMENTAL DESIGN We used a lentiviral-based shRNA kinase library to screen for kinases which play a role in osteosarcoma cell survival. The cell proliferation assay was us...

Osteosarcoma refers to a bone tumor and the term was introduced by John Abernathy in 1804 referring fleshy growth [1]. In 2009, Ottaviane G. et al reported that osteosarcoma is an aggressive malignancy arises from primitive transformed cells of mesenchymal origin produces malignant osteoid, which are most common representation primary cancer [2,3].

The long-term outcomes of osteosarcoma have improved; however, patients with metastases, recurrence or axial disease continue to a poor prognosis. Computer navigation in surgery is becoming ever more commonplace, and the proposed advantages, including precision during surgery, particularly applicable field orthopaedic oncology challenging areas such as skeleton. Within this article, we provide ...

While treatments for childhood osteosarcoma have improved, the overall survival for this common type of bone cancer has not changed for three decades, and thus, new targets for therapeutic development are needed. To identify tumor-related proteins in osteosarcoma, we used isobaric tags in a relative and absolute quantitation proteomic approach to analyze the differentially expressed proteins be...

This study explored the role of fibulin-4 in osteosarcoma progression and the possible signaling pathway involved. Fibulin-4 mRNA and protein expression in normal tissue, benign fibrous dysplasia, osteosarcoma, osteosarcoma cell lines, the normal osteoblastic cell line hFOB, and different invasive subclones were evaluated by immunohistochemistry (IHC) or immunocytochemistry (ICC) and real-time ...

Osteosarcoma is a malignant osteosarcoma that extremely harmful to human health. Magnetic resonance imaging (MRI) technology one of the commonly used methods for examination osteosarcoma. Due large amount MRI image data and complexity detection, manual identification in images time-consuming labor-intensive task doctors, it highly subjective, which can easily lead missed misdiagnosed problems. ...

Abnormal expression of microRNAs plays important functions in osteosarcoma. The aim of this study was to investigate expression, functions and molecular mechanisms of microRNA-363 in osteosarcoma. Quantitative Real-time PCR was used to detect the expression level of microRNA-363 in osteosarcoma tissue samples and cell lines. After transfection, CCK8 assay, cell migration and invasion assay, wes...

Osteosarcoma is the most common primary bone tumour. Increasing evidence has demonstrated the pathogenic role of microRNA (miRNAs) dysregulation in tumour development. miR-379 was previously reported to function as an oncogenic or tumour-suppressing miRNA in a tissue-dependent manner. However, its function in osteosarcoma remains unknown. In this study, we found that the expression of miR-379 w...

introduction: osteosarcoma is the most common primary bone tumor in children and young adults and appropriate chemotherapy can increase limb sparing and overall survival. yet, the toxicity of chemotherapy regimens including mtx can be life threatening. therefore; we tried another chemotherapy regimen for these patients. method and materials: we investigated 15 patients aged 15 to 40 years old a...

background: osteosarcoma is the most common bone sarcoma, and the third most common malignancy in children and adolescents. before 1970, amputation was the sole treatment. eighty percent of patients died from metastatic diseases, most commonly in the lungs. over the past three decades, effective neoadjuvant (preoperative) and adjuvant (postoperative) chemotherapy protocols have improved the abi...