In a recent study (1 ) of diabetic patients with hemoglobins S and C (Hb S and Hb C), we showed a significant decrease in the proportion of glycosylated Hb, as measured by cation-exchange chromatography. We suggested ( I ) that these low values for glycosylated Hb might be caused by the inability of either Hb S or Hb C to undergo glycosylation or by their failure to be eluted from the column wi...

Chromatographic techniques have recently been introduced which provide new approaches to serum protein separation and characterization (1-4). Sober, Gutter, Wyckoff, and Peterson, using diethylaminoethyl (DEAE) cellulose, described the initial application of anion-exchange cellulose chromatography to the fractionation and identification of serum proteins (1, 2). Their study indicated the great ...

This Application Note shows how to carry out qualitative and quantitative analysis of fat soluble vitamins from two different food matrixes. A robust reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous determination of nine fat soluble vitamins was developed. Separation and quantification was achieved by an Agilent 1260 Infinity LC System using an Agilent Por...

The present study involves the optimization of different solvent extract and Standardization of α-mangostin from fruit hulls of Garcinia mangostana was studied by gradient reverse-phase high-performance liquid chromatographic (HPLC) method for quality control and quantity determination. Chromatographic separation was carried out on Develosil RP C-18 (5 μm, 4.6 x 150mm) at room temperature using...

The nitrate-selective copperised-cadmium (Cu-Cd) reduction reaction coupled directly to the highly sensitive nitrite ion chromatographic detection, produced a more precise method for determination of nitrate than any one of the two conventional methods. A borate buffer solution used in the reduction reaction, in place of the conventional ammonium-EDTA buffer solution, eliminated interferences f...

The objective of the current study was to develop a validated, specific and stability-indicating reverse phase liquid chromatographic method for the quantitative determination of fluocinonide and its related substances. The determination was done for active pharmaceutical ingredient and its pharmaceutical dosage forms in the presence of degradation products, and its process-related impurities. ...

A simple, precise, and accurate stability indicating gas chromatographic method has been developed and validated for assay of Magnesium Valproate. The Chromatographic system used to perform development and validation of this assay method consisted of Shimadzu 2014 G.C. with Flame Ionization Detector and a manual injector. The method was validated for specificity, linearity, precision, accuracy,...

We present an accurate, fast, simple and non-destructive photographic method to estimate wax ester and lipid content in single individuals of the calanoid copepod genus Calanus and test this method against gas-chromatographic lipid measurements.