One of serious problems of preparative carbohydrate chemistry is the separation of aldoses from ketoses. Of a large number of chromatographic procedures for separation of aldoses and ketoses described in the literature, only the use of anion exchangers in the bisulfite form afforded satisfactory resolution. Samuelson and Sjoström [1] separated fructose from glucose and mannose on a Dowex 2 ion-...

The advent of Waters UltraPerformance LC® (UPLC®) technology has enabled chromatographic separations to be routinely performed on columns packed with sub-2 μm particles at high linear velocity. Such separations result in high resolution, excellent throughput, and high sensitivity chromatographic analyses.1-2 These analytical qualities ensure the separation of coeluting metabolites and minimize ...

low molecular weight organic acids are most predominant in apple juice among these components fumaric acid was not exceed more than 5 ppm. it was thought that the hplc is the best method of the determination of organic acids in the apple juice. in this study, chromatographic separation of organic acids of apple juice was obtained by preparing a sample and by applying them to acid phase for extr...

Halal food products are requisite to be consumed by Muslim communities in the world. The standard methods capable of quantifying non-halal components very urgent. This review highlights chromatographic and chemometric or multivariate data analysis that offer reliable techniques provide separation capacity halal authentication analysis. article was written from reputable worldwide databases incl...

The packed column supercritical fluid chromatography has risen as a promising alternative separation technique to the conventional liquid and gas chromatography. Although many advantages compared other chromatographic techniques, its mechanism is not fully understood due complex combination effects of parameters on quality lacking global strategies for studying mechanisms. This review aims prov...

a simple and sensitive high performance liquid chromatographic method with electrochemical detection is described for the quantitative determination of the amoxycillin and clavulanic acid in pharmaceutical dosage forms. separation of sample components occurs on a reversed phase c18 column with a mobile phase consisting of methanol-water-phosphate buffer. for the amperometric detection, the pote...

an efficient and simple one-pot approach for the synthesis of 3,4-dihydropyrimidin-2-(1h)-one derivatives using antimony trichloride (sbcl3) as a mild catalyst by means of three-component biginelli reaction between β-keto esters, aldehyde derivatives and urea/thiourea under thermal and solvent-free conditions with excellent yields and short reaction times is reported. this methodology offers se...

A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L-1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separ...

Middle-down mass spectrometry (MS) combined with electron capture dissociation (ECD) represents an attractive method for characterization of proteins and their post-translational modifications (PTMs). Coupling online chromatographic separation with tandem mass spectrometry enables a high-throughput analysis, while improving sensitivity of the electrosprayed peptides and reducing sample amount r...