Cell-free microRNAs in plasma and serum have become a promising source of biomarkers for various diseases. Despite rapid progress in this field, there remains a lack of consensus regarding optimal quantification methods, reference genes, and quality control of samples. Recent studies have shown that hemolysis occurring during blood collection has substantial impact on the microRNA content in pl...

Background: Plasma or serum zinc concentration (PZC or SZC) is the primary measure of zinc status, but accurate sampling requires controlling for hemolysis to prevent leakage of zinc from erythrocytes. It is not established how much hemolysis can occur without changing PZC/SZC concentrations.Objective: This study determines a guideline for the level of hemolysis that can significantly elevate P...

Reducing hemolysis has been one of the major goals of rotary blood pump development and in the investigational phase, the capability of hemolysis estimation for areas of elevated shear stresses is valuable. The degree of hemolysis is determined by the amplitude of shear stress and the exposure time, but to date, the exact hemolytic behavior at elevated shear stresses and potential thresholds fo...

A case of fatal oral poisoning with formic acid resulting in shock, metabolic acidosis, and hemolysis is reported. The formic acid concentration on admission was 348 micrograms/mL, which, together with an increase in lactic acid, contributed to the metabolic acidosis. Because it has been suggested in the literature that formic acid might induce hemolysis via a direct cytotoxic action on the RBC...

The major source of errors producing unreliable laboratory test results is the pre-analytical phase with hemolysis accounting for approximately half of them and being the leading cause of unsuitable blood specimens. Hemolysis may produce interference in many laboratory tests by a variety of biological and analytical mechanisms. Consequently, laboratories need to systematically detect and reliab...

Differential Diagnosis of Hemolytic Anemia Hemolytic anemia reflects shortened RBC life span due to intravascular, extravascular, or fragmentation hemolysis. Intravascular hemolysis is primarily associated with type 1 hypersensitivity reactions, either idiopathic or secondary to another primary disease. In some cases an infectious intraerythrocytic or epierythrocytic agent or miscellaneous diso...

Hemolysis in the GYRO centrifugal blood pump, under development at the Baylor College of Medicine, Houston, TX, is numerically predicted using the newly proposed tensor-based blood-damage model, as well as a traditional model. Three typical operating conditions for the pump are simulated with a special-purpose finite element-based flow solver, and a novel approach for tracing the pathlines in d...

The kinetics of the hemolysis induced by filipin is of the damage type, indicating the formation of large nonselective perforations of erythrocyte membranes. The process is relatively independent of the ionic composition of the incubation medium, and the differences between the hemolysis induced by filipin in pig and human erythrocytes are not significant. In a sucrose medium, filipin-induced h...

BACKGROUND Immune-mediated hemolytic anemia (IMHA) is the most common hematologic immune-mediated disease in dogs. Complement fixation on erythrocytes causes hemolysis. Complement inhibition decreases hemolysis in people with the hemolytic disease and also may prove effective in treating IMHA in dogs. HYPOTHESIS/OBJECTIVES Evaluate the in vitro efficacy of 2 complement inhibitors used in huma...

The kinetics of the filipinand amphotericin-B-induced hemolysis of pig, horse and human erythrocytes in isotonic KCl, monoand disaccharide media was investigated. The kinetics of filipininduced hemolysis is of the damage type, with small changes in erythrocyte resistance to the antibiotic as the standard KCl medium is replaced by the saccharide media. The hemolysis induced by amphotericin B is ...