Filamentous fungi have received attention as hosts for heterologous protein production because of their high secretion capability and eukaryotic posttranslational modifications. However, despite these positive attributes, a bottleneck in posttranscriptional processing limits protein yields. The vacuolar protein sorting gene VPS10 encodes a sorting receptor for the recognition and delivery of se...

Four derivatives of pepstatin, each of which contains the unusual amino acid 4-amino-3-hydroxy-6-methylheptanoic acid (statine) have been prepared. All four are porcine pepsin inhibitors. Both N-acetylstatine and N-acetyl-alanyl-statine are competitive inhibitors for pepsin with Ki values of 1.2 X 10(-4) M and 5.65 X 10(-6) M, respectively. The Ki values for N-acetyl-valyl-statine is 4.8 X 10(-...

By establishing the renal suppletion procedure, the patients with chronic renal failure also have an acceleration of arterial hypertension phenomena. The management of this situation calls for the understanding of the ethological mechanisms (hypervolemia by the reduction of Na excretion and high rennin secretion) and the adaptation of the therapeutic approach to every patient. An individualized...

Food consists of a complex group of substances that differ in chemical composition and physical structure. Collectively these substances form a matrix and it is the behaviour of this matrix which determines the digestibility, the rate and extent of nutrient release, from a food during normal gut transit. In the process of digestion both chemical and physical degradation of food occurs. Chemical...

Cheese production is relied upon the action of rennet (a mixture of chymosin and pepsin) onto casein micelles of milk. For the first time, the monitoring of this interaction with electrochemical impedance spectroscopy (EIS) was used to develop a faradic impedimetric biosensor for the assessment of the clotting activity of rennet, using hexacyanoferrate(II)/(III) couple as a redox probe. Gold el...

At birth, the gastrointestinal tract (GIT) must be able to cope with the shift from parenteral nutrition before birth (via the placenta) to enteral nutrition after birth (oral colostrum/milk intake). In preparation for this event, the GIT grows and matures very rapidly in the weeks before birth. A series of studies in foetal pigs and sheep have shown that both hormonal and luminal factors influ...