ion channels play a major role in the regulation of t cell function in health and disease. in a computer-based model, established to simulate t cells’ membrane potential (vm) generation, we discovered a discrepancy between the simulation and patch-clamp recordings. the predicted vm was more hyperpolarized than the measured vm, indicating that a yet unknown, depolarizing ion current might contri...

One of the common concrete channels in irrigation networks is the semielliptical prefabricated channels. Manning formula is usually used to design these channels. The cross-sectional area and the wetted perimeter are required to be calculated in Manning formula. There are no analytical solutions to directly compute these parameters. Thus, numerical integration methods are inevitably used. In th...

The lipid bilayer is important for maintaining the integrity of cellular compartments and plays a vital role in providing the hydrophobic and charged interactions necessary for membrane protein structure, conformational flexibility and function. To directly assess the lipid dependence of activity for voltage-gated sodium channels, we compared the activity of three bacterial sodium channel homol...

the goal of this study was to determine the effects of the l-type calcium channel blockers verapamil and diltiazem on the currents of voltage-gated potassium channel (fkv1.4δn), an n-terminal-deleted mutant of the ferret kv1.4 potassium channel. measurements were made using a two electrode voltage clamp technique with channels expressed stably in xenopus oocytes. the fkv1.4δn currents displayed...

introduction: our previous studies showed that hydroalcoholic extract of leaf of vitis vinifera relaxes the phenylephrine-induced contraction in rat thoracic aorta. this effect was dependent on endothelial integrity and no-cgmp system. the vasorelaxant effect of extract was much lesser on kcl-induced contraction. we, therefore, postulated that k+ channels are involved. the main aim of the prese...

Precise information regarding the transmembrane topology of mitochondrial porin is essential for understanding the mechanisms by which this protein functions. Porin acts as a channel in the outer membrane and interacts with small solutes and proteins to regulate mitochondrial function. The acquisition of high-resolution structural data requires a method of maintaining high concentrations of una...