Basil (Ocimum basilicum) is one of the important medicinal plant species. In order to produce an autotetraploid population of basil (Ocimum basilicum) by colchicine, different concentrations (0.00, 0.05, 0.10, 0.20, 0.50 and 0.75%) and four treatment methods were examined (seed, the growing point of seedlings at the emergence of cotyledone leaves stage and emergence of true two type leaves stag...

There were no significant differences (age at onset, initial dose of colchicine and serum amyloid A levels) in the two groups. The FMF patients with elevated proteinuria showed a short time between the age of onset and the starting of colchicine than ones with normal proteinuria. Two patients with pathological proteinuria did not have MEFV gene mutations. After the increase of colchicine, there...

Treatment of Nitella antheridia with colchicine results in various sperm abnormalities, depending upon duration of exposure and subsequent recovery. Early effects of treatment include disappearance of spindle fibers and a cessation of ordered cell wall formation in dividing cells. Sperm released from antheridia treated for 24 hr and allowed to recover for 4-5 days possess branched flagella. Aft...

An efficient microspore culture protocol was used to rapidly produce homozygous doubled haploid (DH) progeny of crosses between low erucic canola and high erucic resynthesized rapeseed (Brassica napus L.). Microspores of Canadian cultivars ‘Excel’ and ‘Profit’ as well as three F1 hybrids with the resynthetic line RS239 were treated with colchicine immediately after isolation. Flow cytometry was...

Colchicine is an alkaloid that is used clinically in the treatment of arthritic gout. This potent microtubule disrupting agent has also been used extensively as an experimental tool in studies characterizing the role of the cytoskeleton in a variety of cellular processes. Colchicine has also been used as a selective neurotoxin and in animal models of Alzheimer’s disease and epilepsy. Although t...

The disruption of microtubules by treating bovine aortic endothelial cells with 10-7-10-5M colchicine caused apoptosis, as evidenced by DNA laddering and TdT-mediated dUTP nick end labeling fluorescence staining. Colchicine treatment also induced a sustained activation of c-Jun NH2-terminal kinase (JNK) that lasted for ≥12 h. The blockade of JNK activity by using the negative interfering mutant...

The co-infection of Epstein-Barr virus and Cytomegalovirus rarely gains multi-pathogenicity and leads to viral myocarditis. Also, it may lead to progressive heart failure or sudden death. We present a case series of five patients who were monitored for the impact of low-dose colchicine therapy as adjunct to conventional heart failure therapy. Epstein-Barr virus, Cytomegalovirus and other viral ...

Colchicine overdose is uncommon but potentially life threatening. Due to its serious adverse systemic effects, overdose must be recognized and treated. We report a case of an 18-year-old female who ingested 18 mg (~0.4 mg/kg) of colchicine in a suicide attempt. The patient's clinical manifestations included abdominal cramps, vomiting, pancytopenia, hypocholesterolemia, and rhabdomyolysis. Two u...