Two holding media were compared for their effects on total coliform recovery by the delayed-incubation membrane filter procedure. LES-MF holding medium contains tryptone, m-Endo broth, dipotassium hydrogen phosphate, sodium benzoate, sulfanilamide, para-aminobenzoic acid, and cycloheximide (pH 7.0). m-ST holding medium contains ethanol, sodium monophosphate, dipotassium hydrogen phosphate, sulf...

A rapid and sensitive method is described for the detection of bacteria in water and various other natural substrates by the isolation of specific bacteriophage. By the addition of large numbers of the organism in question to the sample, the presence of virulent bacteriophage can be demonstrated in as little as 6 to 8 h. Fecal coliform, total coliform, and total coliphage counts were determined...

This method relies on the RELATIVE COMPARISON between numbers to formulate a diagnosis. (Examples of this are presented in the appendix). The most common misapplication of the method is the formulation of a diagnosis based on only one of these numbers without considering the relative values of the others (a high LPC count does not always indicate a cleaning failure). As indicated in the interpr...

Reactivation or regrowth of fecal coliform bacteria in biosolids has recently become a concern due to knowledge that Class B materials may fail to meet this criterion after storage or even after land application. In this paper, data show the two types of fecal coliform increases that have been characterized: immediate reappearance of large concentrations directly after dewatering; and the rapid...

Fifty milk samples were assessed for prevalence and enumeration of coliform bacteria under refrigeration. Refrigeration exerted profound effect conferring diminished growth of Citrobacter, Enterobacter and Serratia, however, itfavoured Salmonella, E.coli and Klebsiella on other hand. Among them, Salmonella appeared with highest load both in pre-refrigerated (23%) as well as post-refrigerated (4...

BACKGROUND Samples of drinking water were collected directly from the personal water bottles of students at an elementary school in Calgary, Alberta. METHODS Total and fecal coliforms and heterotrophic bacteria were enumerated using membrane filtration and agar plate count methods respectively. RESULTS The Canadian Drinking Water Quality Guidelines (CWQG) criterion was exceeded for total co...