We present here an improved RNA purification method using fast performance liquid chromatography (FPLC) size-exclusion chromatography in place of denaturing polyacrylamide gel electrophoresis (PAGE). The method allows preparation of milligram quantities of pure RNA in a single day. As RNA oligonucleotides behave differently from globular proteins in the size-exclusion column, we present standar...

The structural information in high-dimensional transposable data allows us to write the data recorded for each subject in a matrix such that both the rows and the columns correspond to variables of interest. One important problem is to test the null hypothesis that the mean matrix has a particular structure without ignoring the dependence structure among and/or between the row and column variab...

In this work, we propose a novel extension of pseudo 2D image warping (P2DW) which allows for joint alignment and recognition of non-rectified face images. P2DW allows for optimal displacement inference in a simplified setting, but cannot cope with stronger deformations since it is restricted to column-to-column mapping. We propose to implement additional flexibility in P2DW by allowing deviati...

A novel hybrid organic-inorganic silica-based monolithic column possessing phenyl ligands for reversed-phase (RP) capillary electrochromatography (CEC) is described. The monolithic stationary phase was prepared by in situ co-condensation of tetraethoxysilane (TEOS) with phenyltriethoxysilane (PTES) via a two-step catalytic sol-gel procedure to introduce phenyl groups distributed throughout the ...

The O-linked sugar chains of the human leukocyte cell surface glycoprotein CD45 were released as tritium-labeled oligosaccharides by beta-elimination in the presence of NaB3H4. Mono Q column chromatography revealed that they comprise neutral (64%) and acidic (36%) oligosaccharides, the latter of which were converted to neutral ones by Arthrobacter ureafaciens sialidase treatment. Structural stu...

[1] Observations from both ground-based and satellite instruments show record low column ozone abundance between 20 S and 40 S during 1997. The 1997 monthly averaged column ozone from the Total Ozone Mapping Spectrometer (TOMS) is up to 25 Dobson units (DU) lower than the TOMS climatological mean (1979–1996) and up to 20 DU below the previous record low values. Observations from the Halogen Occ...

1 An introduction to the matrix‐updating problems A general problem of updating rectangular (or square) matrix can be formulated as follows. Let A be an initial matrix of dimension N × M with row and column marginal totals uA = AeM, v A = e ′ NA where eN and eM are N × 1 and M × 1 summation column vectors with unit elements. Further, let u �= uA and v �= vA be exogenous column vectors of dimens...

The article proposes a structural solution for the base of wooden frame column on flat links glued in support part – steel plates welded to plate. results numerical studies column, taking into account parameters adhesive connection with wood and anisotropy wood, are presented. model takes geometric physico-mechanical characteristics plate bonds compound. main verified analytical calculation per...

This study was to investigate the clinical and radiographical outcomes of anterior spinal column reconstruction using structural femoral shaft allografts in osteoporotic patients. Retrospective analyses of medical records, radiographic parameters, and postoperative complications were performed in twenty-one patients who underwent anterior spinal column reconstruction surgery for osteoporotic ve...

In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained ...