Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 x 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulas...

Abstract There are growing demands by the biodiesel companies to get cheap lipase formulation, preferably in liquid forms, which can give an answer their needs. Thus, many commercial or home-produced enzymes being used this process free form. Among these enzymes, Eversa is a formulation derived from Thermomyces lanuginosus designed for enzymatic production. The potential of promising enzyme her...

Xylanase and β -xylosidase are the key enzymes for hemicellulose hydrolysis. To further improve hydrolysis efficacy, high temperature with thermostable hemicellulases showed promise. In this study, xylanase (Xyn) (XynB) genes from Pseudothermotoga thermarum were cloned secretory expressed in Bacillu subtilis . Compared Escherichia coli expression host, B. resulted a 1.5 time increase of enzymat...

OBJECTIVES To describe the characteristics of randomised controlled trials supported by the main non-commercial sources of funding in the United Kingdom between 1980 and 2002. DESIGN Descriptive survey. SETTING Randomised controlled trials funded by the Medical Research Council, NHS research and development programme, Department of Health, Chief Scientist Office in Scotland, and medical res...

Fish sauce production is a very long process and there is a great interest in shortening it. Among the different strategies to speed up this process, the addition of external proteases could be a solution. This study focuses on the effect of two commercial enzymes (Protamex and Protex 51FP) on the proteolysis of two fish species traditionally converted into fish sauce: sardine and anchovy, by c...

The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidi...

In current study, protein hydrolysates were produced using microbial proteases of Alcalase, Protamex and Flavourzyme from by-products (head, skin and viscera) of Alosa (Alosa caspia), a major Clupeonella species in the Caspian Sea. The results indicated that the protein hydrolysate from Alcalase, had the highest protein content (78.91%), protein recovery (80.42%) and degree of hydrolysis (21.06...

Disintegration of microbial cells is a necessary first step for the production of intracellular enzymes and organelIes. With increasing use o f intracellular microbial material in industry and medicine, the cell disruption unit operation is gaining in importance. This review examines the state of the art o f the largescale cell disruption technology and disruption methods o f potential commerci...

The Zymomonas mobilis genes for ethanol production have been integrated into the chromosome ofKlebsiella oxytoca M5A1. The best of these constructs, strain P2, produced ethanol efficiently from cellobiose in addition to monomeric sugars. Utilization of cellobiose and cellotriose by this strain eliminated the requirement for external 1-glucosidase and reduced the amount of commercial cellulase n...

Commercial cellulase complexes produced by cellulolytic fungi contain enzyme activities that are capable of hydrolyzing non-cellulosic polysaccharides in biomass, primarily hemicellulose and pectins, in addition to cellulose. However, xylanase activities detected in most commercial enzyme preparations have been shown to be insufficient to completely hydrolyze xylan, resulting in high xylooligom...