Synaptic plasma membranes, synaptic junctions, and postsynaptic densities have been isolated from rat brain, the proteins resolved by polyacrylamide gel electrophoresis, and the glycoproteins identified. The synaptic junction is composed of a spectrum of polypeptides which range in Mr from 13,000 to 250,000. The overall pattern is similar to synaptic plasma memranes; however, the relative propo...

outer surface, similar protrusion inwards might be expected with isolated walls, whereas a difference in the gel structure of the wall could explain the morphological difference, and also the finding (Burger, 1966) that isolated walls bind little more antibody than do whole bacteria. The inacessibility of new teichoic acid to concanavalin A at the bacterial surface shows that the teichoic acid ...

Concanavalin A yielded a monodiperse pattern in the analytical ultracentrifuge in the pH range 2-5. The sedimentation coefficient, sOZ~,~ in pH 5 acetate buffer containing 0.1 M NaCl (total I’/2 = 0.20) was 4s. At pH 7 and above, a two-peak schlieren pattern was observed, the faster component (about 7s) probably representing a dimer. On the basis of an s~o.~ of 3.9s; a D 20.~ of 5.43 X 10-T cm2...

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...

A ~ t T h e interaction of concanavalin A with a comprehensive series of carbohydrate-bovine serum albumin conjugates was investigated by agar gel diffusion and quantitative precipitation techniques. These studies showed that when conjugated to bovine serum albumin, those sugars which inhibited concanavalin A-polysaccharide interaction formed a precipitate with concanavalin A. The unexpected re...

During the last few years concanavalin A has become the most widely known and intensively investigated phytohemagglutinin. Much of this interest stems from the discovery that this lectin possesses several remarkable biological properties. In addition to its role as a hemagglutinin,' a structural probe,' and a mitogen,"-" concanavalin A has been shown to restore normal growth patterns to transfo...

Friend erythroleukaemic cells can be induced to mature along the erythroid differentiation pathway when an inducing agent such as dimethyl sulphoxide is included in the medium. In the absence of the inducing agent, the 707B line of Friend erythroleukaemic cells is highly agglutinable by the lectins concanavalin A or wheat germ agglutinin. However, 48 h after the induction of differentiation, th...