BACKGROUND Rabbits are currently not a good model for studying diseases of the corneal endothelium because their corneal endothelial cells (CECs) maintain a high proliferative capacity throughout almost all their life. Addressing this particular feature might allow the use of this species for such a purpose. The aim of this study was to evaluate the corneal endothelial injury after intracameral...

Cell-based therapies have been increasingly explored as a potential alternative treatment method of corneal endothelial dysfunction. Up to date, researchers have mainly dedicated enormous efforts to establishing fundamental techniques for culture and characterization of human corneal endothelial cells (HCECs) and human corneal endothelial progenitor cells (HCEPCs). However, in terms of cell del...

PURPOSE To evaluate visual outcomes, endothelial cell density and complications following Descemet's membrane endothelial keratoplasty (DMEK). METHODS This study included 40 consecutive eyes that underwent DMEK for various pathologies involving the corneal endothelium. Best corrected visual acuity (BCVA) and endothelial cell density (ECD) were measured and compared before and 6 months after s...

PURPOSE To quantitatively analyze laser scanning in vivo confocal microscopy (IVCM) images of all corneal layers in contact lens-wearing and noncontact lens-wearing keratoconus patients. METHODS The study population included rigid gas permeable (RGP) contact lens-wearing keratoconus patients (group 1; N = 29), keratoconus patients who did not wear contact lenses (group 2; N = 30), and subject...

OBJECTIVE The lower recommended age of the donor cornea is a controversial matter. Although newborn corneas have a high endothelial cell density, there are anatomical, refractive (myopic shift) and postoperative problems. Two cases are analyzed; one had an atypical refractive result and the other an unexpectedly severe immune response. We also review the use of pediatric donor corneas in penetr...

PURPOSE To determine the influence of age on central corneal thickness (CCT), endothelial cell density (ECD), average cell size, coefficient of variation in cell size, and percentage of regular hexagonal cells; and to estimate the average ECD and CCT in seven age groups. MATERIALS AND METHODS After obtaining informed consent, 211 Caucasian patients (358 eyes) were examined using a noncontact ...

Purpose To assess corneal hydration control across a range of severity of Fuchs' endothelial corneal dystrophy (FECD) by measuring the percent recovery per hour (PRPH) of central corneal thickness after swelling the cornea and to determine its association with corneal morphologic parameters. Methods Twenty-three corneas of 23 phakic FECD patients and 8 corneas of 8 healthy control participant...

Purpose: The evaluation of the corneal endothelium patients with pseudoexfoliation syndrome (PES) by specular microscopy (SM). Method: In this prospective study, two groups were formed. our right eye 50 diagnosed material slit lamp biomicroscopy performed in ophthalmology outpatient clinic and control without any health problems included. Results: There was no difference between patient terms g...

Abstract The objective of this study was to determine the endothelial cell density (ECD) and hexagonality cornea in different regions healthy swine corneal endothelium using specular microscopy. Twenty-four eyeballs from 12 male, 6-month-old Large White pigs (Sus scrofa domesticus) were studied. Contact microscopy performed central, superior, inferior, lateral medial regions. parameters analyse...

Extracellular matrices (ECM) isolated from chick embryo fibroblast and human and rabbit corneal stromal cells induce polarization and elongation of corneal endothelial cells in culture. ECM isolated from rabbit corneal or bovine aortic endothelial cells neither polarize nor elongate corneal endothelial cells in culture. By indirect immunofluorescence, fibronectin is seen as arrays of long fiber...