The purpose of this investigation was to analyze the proliferative behavior of rabbit corneal epithelium and establish if any particular region was preferentially involved in epithelial maintenance. [3H]-thymidine was injected intravitreally into both normal eyes and eyes with partially scraped corneal epithelium. Semithin sections of the anterior segment were evaluated by quantitative autoradi...

Endothelial keratoplasty is a recent shift in the surgical treatment of corneal endothelial dystrophies, where the dysfunctional endothelium is replaced whilst retaining the unaffected corneal layers. To overcome the limitation of donor corneal shortage, alternative use of tissue engineered constructs is being researched. Tissue constructs with intact extracellular matrix are generated using st...

copy, Vesaluoma et al found loss of keratocytes in the anterior layers of the flap 6 months postoperatively that persisted for up to 2 years. Whether this is the result of apoptosis of keratocytes, lack of communication with lost sensory stromal nerves, or something else has not been determined. Mitooka et al, using confocal microscopy, confirmed this finding and also found fewer keratocytes in...

Corneal hydration and transparency still present multiple dilemmas to the inquiring investigator. Transparency is best explained by the oriented-lattice theory of Maurice, but, except for partly adequate electron micrographs, there is no objective evidence for the presence of such a lattice. Further, other transparent ocular structures are not constructed in this way. Relative dehydration and h...

PURPOSE Laser in vivo confocal microscopy noninvasively provides images that are equivalent to high quality histology. We have now applied this technique to identify pathological characteristics of traumatic recurrent corneal erosion (RCE). METHODS Six eyes of six patients with traumatic RCE were studied. Corneas were examined with a slit lamp biomicroscope and with a laser in vivo confocal m...

PURPOSE The phosphoinositide kinase, FYVE finger containing (PIKFYVE) gene has been identified as a gene responsible for fleck corneal dystrophy (FCD). The purpose of this study is to report a novel mutation of the PIKFYVE gene in a Japanese patient with fleck corneal dystrophy. METHODS Slit-lamp microscopy, corneal topography, and optical coherence tomography were performed for the clinical ...

PURPOSE Congenital stromal corneal dystrophy (CSCD) is characterized by stromal opacities that morphologically are seen as interlamellar layers of amorphous substance with small filaments, the nature of which has hitherto been unknown. CSCD is associated with truncating mutations in the decorin gene (DCN). To understand the molecular basis for the corneal opacities we analyzed the expression of...

copy, Vesaluoma et al found loss of keratocytes in the anterior layers of the flap 6 months postoperatively that persisted for up to 2 years. Whether this is the result of apoptosis of keratocytes, lack of communication with lost sensory stromal nerves, or something else has not been determined. Mitooka et al, using confocal microscopy, confirmed this finding and also found fewer keratocytes in...

PURPOSE To study the short-term clinical results of transplanting of cultivated corneal/limbal epithelial cells on human amniotic membrane (AM) for limbal deficiency. DESIGN Noncomparative, retrospective interventional case series. PARTICIPANTS Thirteen eyes of 13 patients with severe limbal deficiency (Stevens-Johnson syndrome in eight eyes, ocular cicatricial pemphigoid in three eyes, and...

We examined immunohistochemically the localization of three transport enzymes (carbonic anhydrase, Ca-II; sodium-potassium-activated adenosine triphosphatase, NaK-ATPase; bicarbonate-chloride exchanger, band III) in the anterior segment of the human eye. In accord with earlier studies, NaK-ATPase was primarily found in the corneal endothelium, but also in the corneal basal epithelial cell membr...