In this study, the formation of the corticotectal projection of the rat in organotypic slice culture was investigated, using both anatomical and physiological approaches. The establishment of fibre connections from visual cortex to superior colliculus explants was monitored after 3, 6, 14, 20 and 30 days in vitro by cortical injections of Dil. As in cortical cultures without cocultured collicul...

Cortical neurons consist of excitatory projection neurons and inhibitory GABAergic interneurons, whose connections construct highly organized neuronal circuits that control higher order information processing. Recent progress in live imaging has allowed us to examine how these neurons differentiate during development in vivo or in in vivo-like conditions. These analyses have revealed how the in...

Objective: To reveal the neurotoxicity of Methamphetamine (MA) on cortical neurons in rats and to study the protective effects of Rhynchophylline (Rhy) on MA treated neurons. Methods: The neurotoxicity of MA was measured with MTT assay in primary cultured cortical neurons. The viability of neurons treated MA for 48 h combined with Rhy or ketamine was also tested. Results: MTT assay showed that ...

Transplantation of cryopreserved intact ovaries from cancer patients is a technically challenging option for restoring fertility after sterilizing cancer therapy. In this paper we describe an assay based on 17ß-oestradiol (oestradiol) production, to monitor the functional damage sustained by the ovarian tissue during the freeze/thawing procedure. To this end, fresh bovine ovarian cortical biops...

The sparse connectivity within the striatum in vivo makes the investigation of individual corticostriatal synapses very difficult. Most studies of the corticostriatal input have been done using electrical stimulation under conditions where it is hard to identify the precise origin of the cortical input. We have employed an in vitro dissociated cell culture system that allows the identification ...

The perilipins are a family of polyphosphorylated proteins found exclusively surrounding neutral lipid storage droplets in adipocytes and steroidogenic cells. In steroidogenic cells, the cholesterol ester-rich lipid storage droplets are encoated with perilipins A and C. This study describes the dependence of perilipin levels on neutral lipid storage in cultured Y-1 adrenal cortical cells. The a...

Basal forebrain cholinergic neurons (BFCNs) provide the major subcortical source of cholinergic input to cerebral cortex and play an important role in regulating cortical activity. The present study examined the ability of BFCNs to influence neocortical neuronal growth by examining effects of the presence of BFCNs on certain cortical neurons grown under the controlled conditions of dissociated ...

In this work, the surface of porous titanium samples obtained by space-holder technique was treated with a femtosecond laser to improve their osseointegration. Instrumented micro-indentation and scratch test were implemented evaluate tribo-mechanical behavior modified samples. A detailed study micro-hardness, stiffness, resistance elastic recovery performed. Also, in vitro analysis carried out ...

no abstract

The molecular mechanisms that regulate survival of embryonic neural precursors are still relatively ill-defined. Here, we have asked whether the p53 family member p63 plays any role during this developmental window, focusing on the embryonic cerebral cortex. We show that genetic knockdown of p63 either in culture or in the embryonic telencephalon causes apoptosis of cortical precursors and newl...