BACKGROUND Cotton (Gossypium spp.) is a model system for the analysis of polyploidization. Although ascertaining the donor species of allotetraploid cotton has been intensively studied, sequence comparison of Gossypium chloroplast genomes is still of interest to understand the mechanisms underlining the evolution of Gossypium allotetraploids, while it is generally accepted that the parents were...

Transgenic cotton (Gossypium hirsutum L.) with Bacillus thuringiensis Berliner δ-endotoxin genes has been widely adopted for suppression of lepidopteran pests. To account for inconsistency of the endotoxin gene expression, we developed a polymerase chain reaction (PCR) for checking the purity of transgenic cotton plants. A total of eight cry1Ac genes were aligned for the PCR primer design. A DN...

Cotton (Gossypium spp.) is the unique, most important natural fiber crop in the world that brings significant economic income, with an annual average ranging from $27 – 29 billion worldwide from lint fiber production (Campbell et al., 2010). The worldwide economic impact of the cotton industry is estimated at ~$500 billion/yr with an annual utilization of ~115-million bales or ~27-million metri...

Molecular evolutionary rate variation in Gossypium (cotton) was characterized using sequence data for 48 nuclear genes from both genomes of allotetraploid cotton, models of its diploid progenitors, and an outgroup. Substitution rates varied widely among the 48 genes, with silent and replacement substitution levels varying from 0.018 to 0.162 and from 0.000 to 0.073, respectively, in comparisons...

We employ a detailed restriction fragment length polymorphism (RFLP) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. About 46.2% of nuclear DNA probes detect RFLPs distinguishing Gossypium hirsutum and Gossypium barbadense; and 705 RFLP loci are assembled into 41 linkage groups and 4675 cM. The subgenomic origin (A vs. D) of most, and chromosomal identit...

The unpredictability of cotton yields is a great concern to the cotton industry. High temperatures are considered to be one of the main environmental factors contributing to variable yields in cotton (Gossypium hirsutum L.). This has been attributed to a negative effect on respiration and carbohydrate accumulation, but the evidence for this is lacking. In this study it was hypothesized that hig...