The degree of tubulin polymerization in cotton (Gossypium hirsutum L. cv Acala) cotyledonary tissue was estimated by radioimmunoassay which measured the amount of a tubulin-like factor. It was assumed that the release of this tubulin-like factor indicated depolymerization of microtubules. Exposure to chilling resulted in complete release of the tubulin-like factor. Pretreatment with abscisic ac...

Acytogenetic study was performed on 66 tetraploid cotton cultivars (Gossypium hirsutum L.) and their hybrids. The cultivars studied differed significantly in their chiasma frequency and distribution as well as chromosome pairing indicating their genetic differences. Adjacent and alternate quadrivalents were formed in most of the cultivars. A-A or D-D heterozygote translocations occurred in most...

Ann. Entomol. Soc. Am. 81(6): 950-953 (1988) ABSTRACT A simple root-warming device maintains the plants' roots at a higher temperature than that at which the rest of the plant is held. This permits the host plant to retain turgor, not wilt, and therefore remain suitable for and attractive to phytophagous insects. The technique was demonstrated in a development and fecundity test using the cotto...

Experiments were conducted to determine if pretreatment of cotton (Gossypium hirsutum L.) plants resulted in differential in vitro stabilities of nitrate reductase (NR) activity. Although NR activity declines markedly during the second half of the daily light period, in vitro NR stability is not modified by time of harvest. Phenylmethylsulfonylfluoride, iodoacetamide, and N-ethylmaleimide do no...

This paper presents results from field studies carried out during the 1993-1998 Australian cotton (Gossypium hirsutum L.) seasons to monitor off-target droplet movement of endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) insecticide applied to a commercial cotton crop. Averaged over a wide range of conditions, off-target deposition 500 m ...

Leaf shapes of cotton (Gossypium hirsutum) range from highly divided leaves (okra leaf) to normal leaf shape (Meredith, 1 ). The variation in leaf shape results in differences in canopy architecture and light interception characteristics (Wells and Meredith, 1 ). Heitholt et al. (1 ) described higher yields of okra leaf isolines for a given amount of intercepted radiation, indicating that the o...

The identification of molecular markers linked to leaf curl virus (CLCuV) disease resistance in cotton has the potential to improve both the efficiency and the efficacy of selection in cotton breeding programs. Genetic analysis suggested that CLCuV resistance is controlled by a single dominant gene. In this study, an interspecific F2 population derived from a cross of Gossypium barbadense and G...

ACCORDING to STEPHENS (1950) speciation in the genus Gossypium occurs both by cryptic structural differentiation of chromosomes and by major gene substitution and mutation. In the first paper of this series RHYNE (1958) reported a sizeable reduction of recombination values within linkage groups following a transfer of intact diploid Gossypium linkage groups into allotetraploid G. hirsutum. Thes...

We developed a set of genetic markers specific to the A and D genome types of cotton using representational difference analysis (RDA). These markers produce amplification products with genomic DNA from allotetraploid cotton Gossypium hirsutum. One of the markers is a polymorphic amplified restriction fragment (PARF) - a sequence found in both A and D genomes but differently flanked by restricti...