In this work, using benzo(a)pyrene (BaP) as marker, the analytical merits of on-line flow-injection cloud-point extraction (FI CPE), including preconcentration factor, extraction efficiency, sample throughput, and analysis time were evaluated by use of peroxyoxalate chemiluminescence (CL) detection. Moreover, by detailed discussion of several preconcentration conditions for traditional and on-l...

HIV escape in the central nervous system (CNS) despite undetectable viral load in the plasma has been observed and may contribute to HIV-associated neurocognitive disorders. Favouring the use of HIV drugs with a good penetration into the CNS has been advocated, leading to the establishment of the CNS penetration-effectiveness (CPE) score. However, the relevance of this score is not fully establ...

Clostridium perfringens is a leading cause of bacterial food-borne illness in countries where consumption of meat and poultry is high. For example, each year in the United States, this organism is the second or third most common cause of confirmed cases of food-borne illness. Surveys of the incidence of this organism in retail foods were done in the 1960s without regard to whether isolates were...

Previous studies showed that Clostridium perfringens type D animal disease strain CN3718 uses NanI sialidase for adhering to enterocyte-like Caco-2 cells. The current study analyzed whether NanI is similarly important when type A and C human intestinal disease strains attach to Caco-2 cells. A PCR survey determined that the nanI gene was absent from typical type A food poisoning (FP) strains ca...

We evaluated the performance characteristics of chromID CARBA and HardyCHROM Carbapenemase for the detection of carbapenemase-producing Enterobacteriaceae (CPE). A CPE prevalence study was conducted using chromID CARBA; this demonstrated that in low-prevalence settings, CPE screening agars may lack specificity, and confirmation of putative isolates is necessary.

Abstract Great efforts have been made to limit the transmission of carbapenemase-producing Enterobacteriaceae (CPE), however, intestinal reservoir these strains and its modulation by various antibiotics remain largely unexplored. Our aim was assess effects antibiotic administration (ampicillin, ceftazidime, ciprofloxacin) on establishment elimination colonization with a CTX-M-15 ESBL OXA-162 ca...

Background Dysregulated inflammation is associated with many skeletal diseases and disorders, such as osteolysis, non-union of fractures, osteonecrosis, osteoarthritis orthopaedic infections. We previously showed that continuous infusion lipopolysaccharide (LPS) contaminated polyethylene particles (cPE) caused prolonged impaired bone formation. However, the metabolic bioenergetic processes are ...

Carboxypeptidase E (CPE) has recently been described as a neuroprotective protein, and in mice devoid of CPE, a complete loss of the hippocampal CA3 neurons is observed. The pattern of loss is characteristic of that caused by status epilepticus. We therefore set out to determine when this loss occurred, what might induce it and if it could be prevented. We found that the hippocampus was intact ...

12.8% in Greece and 7% in Israel; 27% acquired colonization during ICU stay occurred in the hospital in Israel. A low prevalence of CPE enteric carriage upon ICU admission was observed in this study, but important individual hospital variations in the incidence of ICU-acquired CPE were observed. Four hospitals (36%) had an incidence rate higher than 3%; in three of them nosocomial dissemination...

Carboxypeptidase E (CPE) is initially synthesized as a larger precursor containing an additional 14-residue propeptide that is highly conserved between human and rat. Previous studies have established that the proenzyme is enzymically active and that deletion of the pro region does not affect the expression of the active enzyme. In the present study the function of the pro region was examined b...