Objective Optimum recovery can prevent the athlete's functional capacity from falling between training sessions or competitions. The aim of this study was to evaluate effect dry cupping therapy and creatine supplementation on lactic acid, lactate dehydrogenase plasma kinase following Wingate anaerobic test in male handball players. Methods In quasi-experimental study, 12 young players 4 stages ...

Creatine kinase (CK; EC 2.7.3.2) catalytic activity in serum is widely measured in clinical chemistry practice and provides information for diagnosis and follow-up in many pathological conditions affecting heart, muscle, and brain. Depending on the organ involved, the predominant CK isoenzyme in serum varies. However, routine methods measure total CK catalytic activity, and standardized methods...

We studied the transient appearance of creatine kinase (EC 2.7.3.2) isoenzyme BB, as measured by electrophoresis, in serum or plasma from 19 patients who had just experienced cardiac or respiratory arrest. Creatine kinase BB activity was greatest 0.5 to 3 h after the arrest, with values (measured at 30 degrees C) ranging from 3 to 27 U/L (mean, 7.8 U/L) in 18 patients who were successfully resu...

Human ubiquitous mitochondrial creatine kinase (uMtCK) is responsible for the regulation of cellular energy metabolism. To investigate the phosphoryl-transfer mechanism catalyzed by human uMtCK, in this work, molecular dynamic simulations of uMtCK∙ATP-Mg2+∙creatine complex and quantum mechanism calculations were performed to make clear the puzzle. The theoretical studies hereof revealed that hu...

Arginine and creatine kinase activities in different muscles are compared with calculated maximum rates of ATP turnover. The magnitude of the kinase activities decreases in the following order: anaerobic muscles and vertebrate skeletal muscles greater than heart muscle greater than insect flight muscle. The maximum activity of phosphagen kinases (i.e. creatine kinase and arginine kinase), in th...

Introduction Serum creatine kinase (CK) was first used as a diagnostic aid in progressive muscular dystrophy in 1959 by Ebashi et al (1). It has since become an important Clinical marker for muscle damage. The serum CK level in healthy individuals depends on age, race, lean body mass and physical activity (2, 3). Musculoskeletal disorders often accompany thyroid dysfunction. In addition to the ...

The course of refolding and reactivation of urea-denatured creatine kinase (ATP; creatine N-phosphotransferase, EC 2.7.3.2) has been studied in the absence and presence of molecular chaperonin GroEL. The enzyme was denatured in Tris–HCl buffer containing 6 M urea for 1 h. In the refolding studies, the denatured enzyme was diluted 60-fold into the same buffer containing GroEL or not for activity...