Volatile fatty acids (VFAs) have become promising candidates for replacing the conventional expensive carbon sources used to produce polyhydroxyalkanoates (PHAs). Considering inhibitory effect of VFAs at high concentrations and influence VFA mixture composition on bacterial growth PHA production, a thorough investigation different cultivation parameters such as (synthetic waste-derived VFAs) me...

Oxygen supply implies higher production cost and reduction of maximum theoretical yields. Thus, generation fermentation products is more cost-effective. Aiming to find a key piece for the (poly)-3-hydroxybutyrate (PHB) as product, here we characterize an acetoacetyl-CoA reductase, isolated from Candidatus Accumulibacter phosphatis-enriched mixed culture, showing (kcatNADH/KMNADH)/(kcatNADPH/KMN...

Alkaline pretreatment and sequential enzymatic hydrolysis of soybean hull were investigated to obtain fermentable sugars for polyhydroxyalkanoates production along with residual glycerol as low-cost carbon sources. Soybean is composed approximately 32% cellulose, 12% hemicellulose, 6% lignin, 11% protein. was carried out 2% NaOH concentration, 10% (w/v) biomass loading, 60 min incubation time i...