Phospho-dephosphohybrids of rabbit skeletal muscle phosphorylase (EC 2.4.1.1; alpha-1,4-glucan: orthophosphate glucosyl transferase) have been prepared and stabilized by attachment to Sepharose activated by cyanogen bromide. They can be distinguished from phosphorylase a by their sensitivity to inhibition by glucose-6-phosphate and activation by adenosine 5'-monophosphate. Stable hybrids have a...

Paretsky, D. (University of Kansas, Lawrence), C. M. Downs, and C. W. Salmon. Some biochemical changes in the guinea pig during infection with Coxiella burnetii. J. Bacteriol. 88:137-142. 1964.-Guinea pigs infected with Coxiella burnetii, the rickettsial agent of Q fever, were studied for 11 days postinfection. Maximal changes in liver lipids, liver phosphorylase, and uridine diphosphate glucos...

Arabinogalactan proteins (AGPs) are a family of highly glycosylated, hydroxyproline-rich glycoproteins implicated in various aspects of plant growth and development. (beta-D-glucosyl)3 and (beta-D-galactosyl)3 Yariv phenylglycosides, commonly known as Yariv reagents, specifically bind AGPs in a non-covalent manner. Here (beta-D-galactosyl)3 Yariv reagent was added to Arabidopsis thaliana cell s...

The distribution of immunoglobulin A1 (IgA1) and IgA2 antibodies to various microbial antigens was determined in human parotid saliva by using monoclonal antibodies to the IgA subclasses in a solid-phase radioimmunoassay. In 12 subjects examined, antibodies to Streptococcus mutans glucosyl transferase, protein antigen I/II, and cell wall carbohydrate, as well as dextrans B1355 fraction S and B5...

In the crystal structure of the title compound, C(21)H(25)NO(11), the hexopyranosyl ring adopts a chair conformation and the five substituents are in equatorial positions. An intra-molecular hydrogen bond between the amino group and a neighbouring carbonyl group is found. Two carbonyl groups are disordered and were refined using a split model.

The endoplasmic reticulum (ER) is the major site for folding and sorting of newly synthesized secretory cargo proteins. One central regulator of this process is the quality control machinery, which retains and ultimately disposes of misfolded secretory proteins before they can exit the ER. The ER quality control process is highly effective and mutations in cargo molecules are linked to a variet...