Exogenous C(2)-ceramide has been shown to inhibit polymorphonuclear leukocyte (PMN) phagocytosis through inhibition of phospholipase D (PLD) and downstream events, including activation of extracellular signal-regulated kinases 1 and 2, leading to the hyphothesis that the sphingomyelinase pathway is involved in termination of phagocytosis. Here it is postulated that increased PLD activity genera...

Cloning of embryos by nuclear transplantation has been developed in several species using foetal fibroblasts as donor cells. In the present study, the developmental potential of foetal fibroblasts, arrested in metaphase stage using cytochalasinB, was evaluated using nuclear transfer. Studies were undertaken to find out suitable stage of the cell cycle of goatskin fibroblast cells with enucleate...

Standard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) test is recommended genotoxicity testing, but cytochalasin-B (Cyt-B) may interfere with nanoparticles (NP), leading to inaccurate results. Our objective was determine whether Cyt-B could MN i...

The cytochalasins are a new group of compounds which have been recently isolated from moulds. Some of their properties are of interest in the study of cytoplasmic and nuclear division since they have been shown to inhibit motility, produce nuclear extrusion and prevent cytoplasmic cleavage with the production of multinucleated cells. The present study is a report of the effects of cytochalasin ...

Cytochalasin B, a fungal metabolite, has been shown to inhibit hexose transport in a variety of cellular types, including erythrocytes (Bloch, 1973), leucocytes (Zigmond & Hirsch, 1972), adipose-cell ‘ghosts’ (Czech et al., 1973), fibroblasts and liver cells (Kletzien et al., 1972) and HeLa cells (Mitzel & Wilson, 1972). Studies of effects of cytochalasin B on hexose transport in the nervous sy...