Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.

Cells of Bacillus megaterium can use the externally added precursors UDP-N-acetyl-D-glucosamine and UDP-N-acetylD mU!‘aIUyl L danyl D ghItamy1 mes o diaminopimelyl D alanyl-n-alanine to synthesize peptidoglycan if the bacteria were first treated with toluene. Toluene-treated bacteria could polymerize these precursors into peptidoglycan which was attached to previously existing cell wall by pept...

The specific capsular polysaccharide of type 46 Streptococcus pneumoniae (American type 73) was isolated in pure form and shown to be a high-molecular-weight, glycosidically linked polymer composed of d-galactose (2 mol), N-acetyl-d-glucosamine (1 mol), N-acetyl-d-galactosamine (1 mol), and N-acetyl-l-fucosamine (1 mol).

A covalently triggered fluorescence turn-on detection scheme has been implemented for a tris(N-salicylideneamine)-derived dynamic fluorophore. Selective cleavage of strategically placed Si-O bonds by fluoride ion induces spring-loaded conformational transitions that are tightly coupled to fluorescence enhancement.

The rigid layer and peptidoglycan fractions from two strains (ATCC 17100 and Rm 5) of the budding phototrophic Rhodomicrobium vannielii were isolated. Rigid layers of both strains con­ tain protein in addition to peptidoglycan. They were free of polysaccharides and fatty acids. The respective peptidoglycan fractions contain glucosamine, muramic acid, l and D-alanine, D-glutamic and m eso-diamin...

Aerobic and anaerobic bacteria isolated from human axillae were tested for their capacity to adhere to buccal epithelial cells, immortalized human epithelial (HEp-2) cells, and undifferentiated and differentiated human epithelial cells. In general, both aerobic and anaerobic diphtheroids adhered better to differentiated human epithelial cells than to HEp-2 and undifferentiated human epithelial ...

The causative agent of the pandemic identified as SARS-CoV-2 leads to a severe respiratory illness similar SARS and MERS with fever, cough, shortness breath symptoms cases that can often be fatal. In our study, we report findings based on molecular docking analysis which could new effective way for controlling virus additionally, another manipulative possibilities involving mimicking immune sys...

A novel electrophoretic microchip method for analyzing alpha- and beta-d-glucosamine and their interconversion in solution is presented. d-Glucosamine is labeled with fluorescamine and analyzed by capillary electrophoresis in under 2 min revealing its pH-dependent mutarotation between the alpha- and beta-anomers. The forward interconversion rates for the labeled sugars, based on an iterative an...

We previously reported that glucosamine and hyperglycemia attenuate the response of cardiomyocytes to inositol 1,4,5-trisphosphate-generating agonists such as ANG II. This appears to be related to an increase in flux through the hexosamine biosynthesis pathway (HBP) and decreased Ca2+ entry into the cells; however, a direct link between HBP and intracellular Ca2+ homeostasis has not been establ...