The genes encoding the NADH dehydrogenase subunits of respiratory complex I have not been identified so far in the mitochondrial DNA (mtDNA) of yeasts. In the linear mtDNA of Candida parapsilosis, we found six new open reading frames whose sequences were unambiguously homologous to those of the genes known to code for NADH dehydrogenase subunit proteins of different organisms, i.e., ND1, ND2, N...

Lees are a winery by-product with fiber-rich composition that could have potential prebiotic effect on gut microbiota. Prebiotics cannot be digested by humans but can used bacteria found in the large intestine. To evaluate of lees, they were administered to Wistar rats for 14 days. Feces collected daily, and DNA was extracted analyzed shot gun sequencing. The supplementation lees did not affect...

Yeast communities associated with sugarcane leaves, stems and rhizosphere during different phases of plant development were studied near Campos, in Rio de Janeiro, Brazil. Atmospheric temperature, soil granulometry and pH, and sugar cane juice degree Brix and pH were determined. Yeast communities associated with sugarcane were obtained after cellular extraction by shaking, blending and shaking ...