This study was carried out to determine the effects of supplementation of the maturation media with insulin on in vitro maturation and fertilization of bovine oocytes. In Experiment 1, cumulus-intact bovine oocytes were cultured in a maturation medium (TCM-199 containing 10% fetal calf serum, 0.02 U/ml follicular stimulating hormone and 1 microgram/ml estradiol-17 beta) with or without insulin ...

This study was carried out to determine the effects of supplementation of the maturation media with insulin on in vitro maturation and fertilization of bovine oocytes. In Experiment 1, cumulus-intact bovine oocytes were cultured in a maturation medium (TCM-199 containing 10% fetal calf serum, 0.02 Vlml follicular stimulating hormone and 1 pg/ml estradiol-17 f3) with or without insulin supplemen...

OBJECTIVE To compare three different in vitro maturation (IVM) media for immature oocytes. DESIGN Experimental study. SETTING In vitro fertilization laboratory. ANIMAL(S) BDF1 female and male mice. INTERVENTION(S) Retrieval and maturation of cumulus-enclosed germinal vesicle-stage oocytes according to one of three protocols: group A, conventional IVM medium; group B, blastocyst culture ...

A protein-free chemically defined medium for cultivation of pathogenic Leptospira was developed. The medium permitted continued serial subculturing of 9 serogroups (52 strains) of the 12 serogroups (61 strains) tested. Growth was initiated from small inocula, and the growth rate and maximal cell yields were similar to those on serum-containing media. The nutritional requirements of serogroups L...

19 Exometabolomics enables analysis of metabolite utilization of low molecular weight organic 20 substances by soil isolates. Environmentally-based defined media are needed to examine ecologically 21 relevant patterns of substrate utilization. Here, we describe an approach for the construction of defined 22 media using untargeted characterization of water soluble soil metabolites. To broadly ch...

A nutritional study was made of five strains of Bacillus coagulans obtained from various culture collections. These five strains were descendants of two original isolates; three had been derived from one parent culture in years past and the other two were transfers from another parent culture. Therefore, the five cultures should have represented two distinct groups of genetically identical cult...

Ia antigens and two different types of complement (C) receptors appeared on membrane surfaces in a distinct sequence during the maturation of human neutrophils. Taking advantage of the finding that neutrophil celll density increased with maturation, density gradient centrifugation was used to separate neutrophils into fractions that were greatly enriched in cells representing individual stages ...

This paper proposes a conceptual approach to content delivery that addresses emerging media demand trends through cross-layer integration between Virtualised Service Networks (VSNs) and Information Centric Networking (ICN). The transformative effects of software-defined infrastructures are presented and how these technologies can support dynamics needed to deliver personalised, interactive, mob...