We studied the acute (up to 2 hours after reperfusion) effects of localized cortical hypothermia on ischemia-induced dendritic structural damage. Moderate (31°C) and deep (22°C) hypothermia delays, but does not block the onset of dendritic blebbing or spine loss during global ischemia in mouse in vivo. Hypothermic treatment promoted more consistent recovery of dendritic structure and spines dur...

The Golgi technique stain was used to reveal the cellular structure of the neostriatum (nucleus caudatus and putamen) in the guinea pig. The computerised reconstructions were made from Golgi impregnated neurones. On the basis of various criteria, 4 types of neurones were distinguished in the guinea pig neostriatum: 1. The rounded neurones (most numerous) with 5-8 thin dendritic trunks; 2. The t...

Neurons are highly polarized, but the trafficking mechanisms that operate in these cells and the topological organization of their secretory organelles are still poorly understood. Particularly incipient is our knowledge of the role of the neuronal endoplasmic reticulum. Here we review the current understanding of the endoplasmic reticulum in neurons, its structure, composition, dendritic distr...

background: generation of an effective dendritic cell (dc) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. objective: to compare the effects of monocyte separation methods, flask adherence (flask-dc) and magnetic activated cell sorting (macs-dc), on phenotypic and functional characteristics of resultant dcs. methods: dcs from healthy volunteers were generated ...

objective(s)  garlic (allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. one of the major purified garlic protein components is the 47 kda protein. in this study, the effect of 47 kda protein extracted from aged garlic (age) was evaluated on mouse dendritic cell (dc) maturation in vitro. materi...

In the work influence of thermal limiters on formation a dendritic structure stainless steel AISI 304L layer deposited by electric arc welding in argon medium was investigated. The billets investigated mode differential interference contrast. analysis obtained structures showed that use form graphite skids allows to obtain uniform without sharp transitions between layers.

The functional geometry of the reconstructed dendritic arborization of Purkinje neurons is the object of this work. The combined effects of the local geometry of the dendritic branches and of the membrane mechanisms are computed in passive configuration to obtain the electrotonic structure of the arborization. Steady-currents applied to the soma and expressed as a function of the path distance ...

For decades, neuroscientists have marveled at the complexity and intricacy of dendritic trees and pondered their function. At first, it was proposed that dendrites served as a way to increase the surface area of a neuron, allowing it to receive more synaptic inputs. Later, with the emergence of cable theory, dendrites were viewed by some as an inconvenience, due to the loss of current along the...

In vivo and in vitro multichannel field and somatic intracellular recordings are frequently used to study mechanisms of network pattern generation. When interpreting these data, neurons are often implicitly considered as electrotonically compact cylinders with a homogeneous distribution of excitatory and inhibitory inputs. However, the actual distributions of dendritic length, diameter, and the...

Sub-monolayer physical layer deposition of Zn on Ag(111) substrate at room temperature is investigated using Auger electron spectroscopy, low energy diffraction and scanning tunneling microscopy. We identified an original highly-anisotropic structure in the shape finger-like inserted dendrite front monolayer growth. On basis STM observations, a mechanism dendritic growth its transition to layer...