نتایج جستجو برای: dependent probe amplification

تعداد نتایج: 823586  

Journal: :Medicina 2015
Daniela Ottaviani Cristina Alonso Irene Szijan

Retinoblastoma (RB) is the most common primary intraocular malignancy in children. Somatic inactivation of both alleles of the RB1 tumor suppressor gene in a developing retina is a crucial event in the initiation of tumorigenesis in most cases of isolated unilateral retinoblastoma. We analyzed the DNA from tumor tissue and peripheral blood of a unilateral retinoblastoma patient to determine the...

Journal: :The Analyst 2013
Jeongkyeong Na Gi Won Shin Gyu Yong Jung Gyoo Yeol Jung

Aberrant DNA methylation is a potential diagnostic marker for complex diseases, such as cancer. With the increase in the number of genes known to exhibit disease-associated aberrant methylation, the need for accurate multiplex assays for quantifying DNA methylation has increased. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is one method that has been highligh...

2008
Jordy Coffa Mark A. van de Wiel Begoña Diosdado Beatriz Carvalho Jan Schouten Gerrit A. Meijer

BACKGROUND Multiplex Ligation dependent Probe Amplification (MLPA) is a rapid, simple, reliable and customized method for detection of copy number changes of individual genes at a high resolution and allows for high throughput analysis. This technique is typically applied for studying specific genes in large sample series. The large amount of data, dissimilarities in PCR efficiency among the di...

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